中文摘要：

采用荧光定量PCR、Westernblot及免疫组化方法对比研究gankyrinmRNA及蛋白在甲状腺乳头状癌（papillarythyroidcarcinoma，PTC）、桥本氏甲状腺炎（Hashimoto’s thyroiditis，HT）；ZL正常甲状腺组织中的表达情况，分析gankyrin蛋白表达与PTC临床病理特征的关系，探讨gankyrin基因对PTC发生发展的影响。结果显示，PTC及HT样本中gankyrinmRNA及蛋白水平的表达量均高于正常对照组，有统计学显著意义（P〈0．01）；PTC组7LHT组间的表达无统计学差异（P〉0．05）；临床特征分析表明，gankyrin蛋白表达在PTC中的表达与患者的性别、年龄、TNM分期等无关，而与是否合并HT有关。Gankyrin基因在PTc及HT中的高表达说明其在这两种关系密切的疾病中扮演着重要的角色，提示其与PTC的分子机制有关。

英文摘要：

In this research, we evaluated the relationship between gankyrin expression and clinicopathologic parameters in papillary thyroid carcinoma （PTC）, exploring its effect on PTC using fluorescence quantitative PCR, Western blot and immunohistochemistry to investigate mRNA and protein levels of gankyrin in PTC, Hashimoto＇s thyroiditis （HT） and normal thyroid tissues. The results showed that both the mRNA and protein levels of gankyrin expression in PTC and HT were obviously higher than that in normal thyroid tissues （P〈0.01）, whereas the differ- ences of gankyrin expression were not significant between PTC and HT （P〉0.05）. In addition, gankyrin was over- expressed in PTC with coexisting of HT compared with PTC without HT. The high level ofganltyrin expression in both PTC and HT suggests that gankyrin plays an important role in the occurrence and development of these two closely related diseases and it is probably a molecular mechanism of PTC.