中文摘要：

卵巢癌是妇科疾病中仅次于宫颈癌和子宫内膜癌的常见肿瘤，死亡率较高．该肿瘤治疗失败的主要原因之一是复发后的肿瘤细胞凋亡抵抗机制导致化疗的耐药性．在卵巢癌的化疗中顺铂是主要使用的药物之一，但由于细胞多药耐药性的产生严重限制了其疗效的发挥．蛋白质组学是在蛋白水平上对整个器官、细胞或组织的基因组所表达的全部蛋白质进行总体分析，双向凝胶电泳和质谱技术则是研究蛋白后组学的核心技术．

英文摘要：

The proteomic maps of human ovarian adenocarcinoma cell line in Human COC1 and its cell subline COC1/DDP were produced by 2-DE SDS-PAGE. The image analysis revealed obviously differential protein expression of the cells in response to cisplatin treatment. Approximate 1 300 protein spots were resolved in the two ranges of pl（3-10）. The spots which have 10-fold difference in light density were digested by in-gel enzyme, identified by matrix assisted laser desorption/ionization time-of-flight mass spectrometry （MALDITOF-MS） and finally detected by Profound and Mascot search engine in IDI databases. Seven proteins with obviously altered abundances were detected specifically, including four up-regulated protein spots and three down-regulated protein spots. The proteins differentially expressed in the parent cell line COC1 and its subline COC1/DDP were considered to be important for the therapy and pathogenesis.