中文摘要：

设计了基于超声换能器和球面声透镜的超声聚焦微喷头及向下隔离喷射机构,实现了无喷嘴、无堵塞、非接触、低消耗、高精度的微阵列制备。为验证其生物相容性,以载有F0F1-ATP酶的分子马达脂质体为对象,制备出了形状规则、定位准确的脂质体微阵列,并对其ATP水解活性和合成活性进行了检测。实验结果表明,所制备的微阵列生物芯片在相关光化学刺激下具有良好的ATP水解与合成活性,从而证实该方法在微阵列生物芯片制备过程中能够较好地保持样品的生物活性。

英文摘要：

Focusing on the problems existing in the biochip microarray manufacturing methods such as low maintenance in bioactivity,microjet head blockage and high consumption in biological reagent and so on,the ultrasonic transducer and spherical acoustic lens based on ultrasonic focusing microjet head as well as the downward isolated jetting mechanism was designed.Based on this,no nozzle,non blockage,non contact,low consumption and high accuracy was gained in the microarray manufacture.To verify the biocompatibility of the microjet system,the F0F1-ATP molecular-motor assembled liposome microarrays with regular shape and accurate location was prepared.Furthermore,the ATP hydrolysis activity and the ATP synthesis activity of the liposome microarrays were tested respectively.Experimental results indicated that the liposome microarrays fabricated this way showed favorable ATP hydrolysis activity and synthesis activity,which confirmed that the ultrasonic focusing microjet method has favorable biocompatibility in manufacturing biological microarrays.The investigation elaborated here explored a new manufacturing approach for biological microarrays.