中文摘要：

采用改进的SDS法抽提大血藤基因组DNA，分析了镁离子、dNTP、模板DNA含量、引物浓度、Taq DNA聚合酶量和BSA浓度对大血藤ISSR-PCR扩增结果的影响，建立了稳定的、可重复的大血藤ISSR最佳反应体系：10μL PCR反应体积中，4×Taq酶配套缓冲液（10mmol／L Tris-HCl，pH9．0，50mmol／L KCl，1g／L Triton X-100），0．5U Taq DNA聚合酶，2mmol／L MgCl2，0．1mmol／L dNTP，12pmol引物，10ng模板DNA，2mg／mL牛血清白蛋白。大血藤的最适退火温度为52．4℃。

英文摘要：

The genomic DNA of Sargentodoxa cuneata was extracted with improved SDS method. The effect of the concentration of Mg^2＋ , dNTP , DNA template, primer, Taq DNA polymerase and bovine serum albumin on ISSR amplification of Sargentodoxa cuneata was tested and the suitable ISSR reaction system was established as follows： 4 × Taq polymerase corresponding buffer （ 10 mmol/L Tris - HCl , pH9.0, 50 mmol/ L KCl , 1 g/L Triton X -100） , 0.5U Taq DNA polymerase, 2 mmol/L MgCl2, 0.1 mol/L 4 × dNTP, 12 pmol primer , 10 ng template DNA and 2 mg/mL BSA in total 10 μL PCR reaction volume. The suitable annealing temperature in the ISSR - PCR reaction system was 52.4 ℃.