中文摘要：

目的研究异种脱细胞猫角膜板层角膜移植术后的组织相容性。方法24只18周龄的健康新西兰白兔随机平均分成3组：A组为脱细胞猫角膜材料→兔板层移植组,B组为猫→兔异种新鲜角膜植片板层移植组,C组为兔→兔同种异体新鲜角膜植片板层移植组。用扫描电镜观察术后不同时期术眼角膜的组织病理学改变,抽取兔血测定CD4^＋/CD8^＋T细胞百分比。结果术后1个月3个组间角膜植片炎症反应指数组间比较差异均无统计学意义;2个月A组与B组、A组与C组间比较差异均有统计学意义（P〈0.05）。3个月A组与B组、A组与C组间比较差异均有统计学意义（P〈0.05）。3个组间术后同期7、15、30d兔外周血CD4＋T细胞和CD8＋T细胞活化率差异均无统计学意义（P〉0.05）。组织切片显示术后3个月内,B、C两组角膜植片炎症反应轻,植片内皮细胞吸收,组织结构基本正常。A组角膜植片炎症反应显著,结构较紊乱,新生血管增生。扫描电镜可见B、C2组角膜上皮结构基本正常,细胞连接较好。A组扫描电镜可见有上皮缺损。结论脱细胞处理的异种猫角膜移植后生物相容性较新鲜角膜差,免疫反应无显著差异。

英文摘要：

Objective The present study was to explore the histoeompatibility, graft survival time and inflammatory response after xenogeneie acellular cat lamellar keratoplasty onto rabbits. Methods Twenty-four healthy 18-week-old male New Zealand white rabbits were as reeeiptors and randomly divided into three groups：group A received cat-rabbit acellular xenogeneie lamellar keratoplasty; group B underwent cat-rabbit xenogeneic lamellar keratoplasty; group C was rabbit-rabbit corneal allograft lamellar keratoplasty, The diameter of the donor graft was 9 mm and the diameter of receipt bed was 8 mm. The inflammatory response and survival of graft were observed for 3 months. Histological and ultrastructure examination was performed at the 1 st, 2nd and 3rd month under the optical microscope and scanning electron microscope, respectively, and the percentages of CD4^＋/CD8^＋ from rabbit periphery blood were detected at the 7th, 15th and 30th day after the surgery. Results The inflammation index was 2. 38±1. 506,1.75 ± 1. 488 and 1. 25 ± 1. 282 in the 1st month in group A,B and C without significant difference among three groups （ P 〉 0.05 ） , and that in the 2nd month was significantly different between group A and group B（5.83±1.722 vs2.50±1.517） or between group A and group C（5.83±1.722 vs 2. 50±1.378）（P〈0.05）.In the 3rd month,the inflammation index was remarkably higher in group A compared with group B and group C （6.50 ± 0. 774 vs 2. 50±1. 291,6.50±0. 774 vs 2.75±0. 957） （P 〈0. 05）. No significantly differences were found in activation rates of CD4 ＋ T cells and CD8 ＋ T cells upon surgery time and among different groups（P 〉 0. 05） based on two-way ANOVA. Histological sections showed that the inflammatory reaction was more slight in group B and group C than group A three months after operation. Corneal epithelial cells were stratificated and cellular boundary were tightly opposed,but epithelial defect was displayed in group A. Conclusion The bioeompatibility of the acell