双水相萃取技术作为一种新型的绿色分离/富集技术,具有简单、省时、高效和绿色无污染等优点,已被应用于金属离子的定量分离萃取、生物活性物质的分离纯化以及天然产物的提取等领域。目前的双水相体系主要包括聚合物-聚合物双水相体系、聚合物-盐双水相体系、离子液体-盐双水相体系和小分子有机溶剂-盐双水相体系,但由于有机溶剂易挥发不稳定、离子液体成本较高和两种聚合物体系的粘度较大等问题,影响了这三种双水相体系在工业规模化生产中的应用。而聚合物-盐双水相体系用盐代替聚合物-聚合物双水相体系中的一种聚合物作为成相物质,在降低体系粘度和生产成本的同时,保留了聚合物生物相容性好的优势,被广泛应用于生物活性物质、天然产物及抗生素的分离纯化,具有较高的开发价值和广阔的应用前景。通过分析聚合物-盐双水相体系的理论及应用研究进展,希望对进一步的研究工作有所帮助和启发。
Aqueous two phase extration is a new and green separation and enrichment technology,and it has some advantages,such as simple,timesaving,efficient,green and eco-friendly,and it has been applied to the quantitative separation and extraction of metal ions,separation and purification of bioactivator and extraction of natural product. The existent aqueous two-phase system included polymer-polymer aqueous two-phase system, polymer-salt aqueous two-phase system,ionic liquid-salt aqueous two-phase system and micromolecule organic solvent-salt aqueous two-phase system. Because organic solvent is volatile and instable,the price of ionic liquid is higher and the viscosity of system containing two polymer is larger,the application of these three types of aqueous two-phase systems in large-scale industrial production was affected. One polymer of polymer-polymer aqueous two-phase system was replaced by salt,and it is polymer-salt aqueous two-phase system. The cost and viscosity of polymer-salt aqueous two-phase system is cheaper,and it has the advantage of good biocompatibility. It is applied in the separation and enrichment of bioactivator,natural product and antibiotic,and has high-exploited value and broad prospects on its application. In this paper,the research progress on the polymer-salt aqueous two-phase system was given,and hoping that is will be of help fo further research.