中文摘要：

为明确食物中重金属离子在昆虫体内的分布和转移情况,本文采用等离子体原子发射光谱仪检测了植食性昆虫斜纹夜蛾Spodoptera litura Fabricius连续3个世代6龄幼虫对食物中过量Ni^2＋的排泄和积累情况。结果表明：大部分Ni^2＋可通过粪便排出体外;沉积在体内的Ni^2＋主要积累在中肠,部分Ni^2＋可通过中肠上皮细胞基底膜进入血淋巴,经由血淋巴的转运作用积累在脂肪体和表皮等组织中。6龄幼虫不同组织中所积累Ni^2＋的含量为中肠〉脂肪体〉表皮,且不同组织和粪便中的Ni^2＋都随饲料中Ni^2＋浓度的增加而增加,并存在显著的剂量-反应关系。研究结果可为进一步研究过量Ni^2＋对斜纹夜蛾幼虫的生长发育和繁殖的影响,以及斜纹夜蛾幼虫不同组织对Ni^2＋的解毒能力等提供一定依据。

英文摘要：

To study the distribution and transfer of heavy metal in insects, nickel amounts in the faeces and tissues of the 6th instar Spodoptera litura Fabricius larvae fed on artificial diets amended with different concentrations of nickel for 3 generations were detected by Inductively Coupled Plasma-Atomic Emission Spectrometer （ICP-AES）. The results showed that majority of nickel in diets could be excreted through faeces. Nickle uptaken by S. litura larvae was primarily stored in the midgut of larvae, and some of nickel could enter the hemolymph via intestine and then be transferred to fat body and cuticle. Nickel accumulated in the midgut was more than those in the fat body and the cuticle in which nickel accumulation was the lowest. The results also indicated that nickel in faeces and different tissues of S. litura larvae within a generation increased with the increase of nickel concentrations in the treated diets and showed significant dose-dependent relationship with the nickel concentrations in artificial diets. The results might provide insight into the effects of nickel contamination on development and reproduction of S. litura, as well as the detoxification of S. litura to heavy metal nickel.