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空气净化器降低室内尘螨过敏原含量及其免疫反应性的实验研究

ISSN号：1002-2694
期刊名称：《中国人兽共患病学报》
时间：0
分类：R384.4[医药卫生—医学寄生虫学;医药卫生—基础医学]
作者机构：[1]深圳大学医学院过敏反应与免疫学研究所,深圳518060
相关基金：国家863计划项目（No.2006AA02A231）、国家自然科学基金（No81071388、30760082）、深圳市重点实验室组建项目（NoSW201110010）、深圳市科技计划基础研究重点项目（No.2012016）南山区科技计划项目（No.KC20挖JsYBO（）（]3A）联合资助

作者：马忠校[1], 刘晓宇[1], 杨小猛[1], 王媛媛[1], 吴莹莹[1], 刘志刚[1]

关键词：粉尘螨, 空气净化器, 过敏原, Der, f检测, 免疫反应性, dust mites air purifier allergens Derf test immunoreactivity

中文摘要：

目的检测开启空气净化器前后室内空气中粉尘螨主要过敏原的含量，探讨粉尘螨经空气净化器不同作用时间后对其抗原免疫反应性的影响。方法实验分组采样：（1）不开空调和空气净化器；（2）开空调、不开空气净化器；（3）同时开空调和空气净化器；（4）开空气净化器、不开空调。用粉尘采样器分别采集室内空气中尘螨过敏原Derfl和Derf2，运用ELISA方法检测4种环境条件下空气中Derfl、Derf2的含量；通过ELISA和免疫印迹方法检测空气净化器作用不同时间（24h、48h和72h）后粉尘螨过敏原免疫反应性的变化。结果20个取样房间在开空调之后室内尘螨过敏原的含量都会增高，而在使用空气净化器后，不管是在空调开启或者关闭的情况下室内空气中Derfl和Derf2都会降低（P％0．01）；粉尘螨在空气净化器等离子和静电高压场强作用下，死亡的粉尘螨其免疫反应性均较活螨显著降低（P％0．01），免疫组化显示其免疫反应性有所降低。结论本实验使用空气净化器能够显著降低室内空气中的尘螨过敏原Derfl、Derf2的浓度，并且通过空气净化器作用后，死亡的粉尘螨免疫反应性显著降低，提示使用空气净化器对过敏性疾病（如哮喘和过敏性鼻炎）具有重要的预防和干预作用。

英文摘要：

This research aims to detect the indoor allergen concentration before and after air cleaner operating and inves- tigate the effects of it on antigen immunoreactivity as time changing. The samplers of dust mite allergens were collected through dust sampler from the four knids of environments：（1） air conditioner and air cleaner were both turned off; （2） air conditioner was runing and air cleaner was turned off; （3） air conditioner and air cleaner were both runing; （4） air conditioner was turned off and air cleaner was running. The concentrations of dust mite allergens from four kinds of environments were detected by ELISA. Dust mite allergens were treated by air cleaner in different timing points, and through ELISA and immunoblotting, the changes of dust mite allergen immune activities were determined. Results showed that the concentrations of dust mite allergens were increased in all 20 sample rooms when air conditioners were running. However, when the air cleaner was running, the concentrations of Der f 1 and Der f 2 were reduced whatever the air conditioner was operating or not （P d0.01）. The im- mune activities of dust mite allergen were decreased because of plasma sterilization. In conclusion, the air cleaner in this study could reduce the concentrations of dust mite allergens Der f 1 and Der f 2, and also reduce the immune activities of allergens on the filters by plasma sterilization. Our results indicate that air cleaner plays an essential role in prevention of allergic disor- ders such as asthma and allergic rhinitis.

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