中文摘要：

目的：研究胞内信号转导分子LIM矿化蛋白-1在人牙周膜细胞体外矿化过程中的表达。方法：利用组织块法体外培养人牙周膜细胞，实验组培养液中加入矿化诱导剂，对照组不加矿化诱导剂。培养14d后，免疫细胞化学检测骨涎蛋白的表达；von Kossa染色检测矿化结节的形成。培养期间，采用半定量RT-PCR技术监测LIM矿化蛋白-1、碱性磷酸酶的表达，SPSS11．0软件分析结果。结果：培养14d，实验组骨涎蛋白免疫细胞化学呈阳性表达，Yon Kossa染色阳性，矿化结节形成；对照组骨涎蛋白表达阴性，yon Kossa染色阴性。RT-PCR结果显示碱性磷酸酶、LIM矿化蛋白-1在实验组和对照组各时段均表达。培养期间，实验组LIM矿化蛋白-1的表达显著高于对照组，其中培养第3d、14d时表达分别达到两个高峰，约为对照组的1．4倍、1．5倍。培养期间，碱性磷酸酶的表达显著增高，其中培养14d时约为对照组的1．5倍。结论：首次发现LIM矿化蛋白-1与人牙周膜细胞的体外矿化过程相关，提示LIM矿化蛋白-1可能在牙周膜细胞的分化、矿化中发挥一定作用。

英文摘要：

AIM： To investigate the expression pattern of LIM mineralization protein - 1 during mineralization of cultured human periodontal ligament cells. METHODS： Human periodontal ligament cells were differentiated via culturing in the presence of dexamethasone, asorbic acid and β - glycerophosphate for bone - like mineralized nodule formation, characterized by yon Kossa staining and by immunocytochemistry of bone sialoprotein. Expression of LIM mineralization protein - 1 and alkaline phosphatase was analyzed by reverse transcriptase - polymerase chain reaction on day 0, 1,2, 3, 5, 7, 9, 11, 14, 21 and 28. RESULTS： During the mineralization, LIM mineralization protein - 1 and alkaline phosphatase were expressed at every time point, generally held at a statistically significantly higher level compared with the control. For LIM mineralization protein - 1, 1.4 - fold increased on day 3 and 1.5 - fold on day 14 ; for alkaline phosphatase, 1.5 -fold increased on day 14. CONCLUSION： LIM mineralization protein- 1 is found to be involved in the mineralization of human periodontal ligament cells. LIM mineralization protein - 1 may play a role in the differentiation and mineralization of human periodontal ligament cells.