采用营养液培法,以加工番茄品种‘里格尔87-5’为材料,通过对NaCl胁迫下加工番茄幼苗叶片分别喷施5、10、15 μmol·L-1NADP-和NADPH,研究外源NADP+、NADPH对NaCl胁迫下加工番茄幼苗生长及其抗逆生理指标的影响.结果表明:外源喷施NADP+、NADPH均能改善NaCl处理下加工番茄幼苗的生长性状,增加叶片叶绿素含量,提高根系活力和叶片过氧化物酶(POD)、超氧化物歧化酶(SOD)、过氧化氢酶(CAT)和抗坏血酸过氧化物酶(APX)活性,降低丙二醛(MDA)质量摩尔浓度.通过不同处理综合指标总加权值比较评价得出:NaG1胁迫下喷施5μmol·L-1 NADPH的效果最佳,其次为10 μmol· L-1 NADP+.可见,外源喷施NADP+ 、NADPH通过增加色素含量,可提高抗氧化酶活性,降低膜脂过氧化水平,在一定程度上缓解盐胁迫对植株的伤害,从而增强对盐胁迫的适应性.
The objectives of this hydroponic experiment were to determine the effects of exogenous NADP+ and NADPH on the growth of processing tomato (Solanum lycopersicum cv.Ligeer 87-5) seedlings under NaCl stress.The tomato leaves were sprayed with either NADP+ or NADPH at concentrations of 5,10,and 15 μmol · L-1.The results showed that exogenous NADP+ and NADPH reduced malondialdehyde (MDA) mass molality and increased the growth of tomato seedlings under NaCl stress.Exogenous NADP+ and NADPH increased the activities of superoxide dismutase (SOD),peroxidase (POD),catalase (CAT) and ascorbate peroxidase (APX).Exogenous NADP+ and NADPH also increased leaf chlorophyll content and root activities.Comprehensive evaluation of the physiological indexes by subordinate function values analysis indicated that spraying 5 μmol · L 1 NADPH in the NaCl treatment had the best treatment effect,followed by 10 μmol · L ~ NADP+.These results indicated that exogenous NADP+ and NADPH can alleviate the damage of NaCl stress on tomato seedlings by reducing membrane lipid peroxidation and increasing leaf chlorophyll mass fraction and antioxidant enzyme activities.