中文摘要：

本试验旨在研究黄芪多糖（Astragalus polysaccharin,APS）对肉鸡外周血淋巴细胞（peripheral lymphocyte,PLC）与颈静脉内皮细胞（jugular vein endothelial cells,JVEC）间黏附的影响。以4种剂量的APS（0、200、600和1000μg/mL）分别处理肉鸡JVEC和PLC,观察二者间黏附量的变化;以白介素-1（IL-1,1000U/mL）和肿瘤坏死因子-α（TNF-α,1000U/mL）分别与4种剂量的APS（0、200、600和1000μg/mL）共同处理JVEC和PLC,观察不同剂量APS对PLC与JVEC间黏附的影响,及对JVEC表面细胞间黏附分子-1（ICAM-1）和PLC表面CD4^＋变化的调节。结果表明,600μg/mL APS处理JVEC,可显著增加PLC与JVEC间的黏附（P〈0.05）;IL-1与高剂量的APS（1000μg/mL）共同处理JVEC或IL-1与3种剂量的APS（200、600和1000μg/mL）共同处理PLC,均能显著增加PLC与JVEC间的黏附（P〈0.05）;与对照组相比,TNF-α单独处理JVEC后可显著增加PLC与JVEC间的黏附（P〈0.05）;与对照组相比,IL-1与高剂量APS（1000μg/mL）共同处理JVEC后可显著增加JVEC表面ICAM-1值（P〈0.05）,IL-1与高剂量APS（1000μg/mL）共同处理PLC后可显著增加PLC表面CD4^＋阳性细胞百分率（P〈0.05）。综上所述,APS调控PLC与JVEC间黏附受其剂量的影响,在本试验条件下,中、高剂量APS（600和1000μg/mL）单独或与IL-1、TNF-α协同作用可增加肉鸡外周血淋巴细胞与颈静脉内皮细胞间的黏附,同时高剂量APS（1000μg/mL）可通过改变血管ICAM-1和T淋巴细胞亚群CD4^＋的分泌（表达）发挥其免疫调节作用。

英文摘要：

The objective of this experiment was to study the effect of Astragalus polysaccharin （APS） on adhesion of peripberal Iymphocyte （PLC） and jugular vein endothelial cells （JVEC） in broiler. The JVEC and PLC of broiler were treated independently with four doses of APS （0,200,600 and 1000 flg/mL） and the adhesion changes of them were observed subsequently. Also the two types of cells were incubated with interleukin-1 （IL-1,1000 U/mL） or tumor necrosis factor-α （TNF-α, 1000 U/mL） to investigate the effect of APS on intercellular adhesion and the regulatory mechanism of APS to intercellular cell adhesion molecule-1 （ICAM-1） and T-lymphocyte subsets CD4^＋. The results showed that the PLC-JVEC adhesion increased significantly when the JVEC was incubated with APS （600 μg/mL） （P〈0. 05）. The same significant increment of intercellular adhesion was observed when the JVEC was incubated with the combination of IL-1 and APS （1000 μg/mL） ,or the PLC was incubated with the combination of IL-1 and APS （200,600 and 1000 μg/mL） （P〈0. 05）. Compared with the control group, the PLC-JVEC adhesion increased significantly when the JVEC was incubated with the combination of TNF-α （P〈0. 05）. Compared with the control group, the values of ICAM-1 and percentage of positive cell （CD4^＋） increased significantly with the combined treatment of IL-1 and high dose of APS （1000 μg/mL） （P〈0. 05）. In conclusion, the dose of APS affected its regulation on PLC-JVEC adhesion. Under this experimental conditions, both the medium and high doses of APS （600 and 1000 μg/mL） could increase the PLC-JVEC adhesion alone or with the combination of IL-1 and TNF-α. At the same time, the high dose of APS （1000 μg/mL） could exert the immunomodulatory by changing the secretion （expression） of ICAM-1 and CD4^＋.