中文摘要：

脱落酸（ABA）受体RCARs在ABA信号传导过程中起关键作用，研究ABA受体的相互作用蛋白质对进一步了解植物抗逆的分子机制有重要的意义．本研究通过酵母双杂交发现CARK7与ABA受体RCAR12共转化酵母能在营养缺陷型平板SD／-Trp—Leu—Ade上生长．将CARK7与RCAR12分别克隆到原核表达载体pET-28a（＋）和pGEX-6p-1中，构建融合蛋白表达载体，并进行原核表达和亲和纯化．纯化所得蛋白进行GST—pulldown实验，结果表明RCAR12能够把CARK7蛋白拉下来．上述结果说明CARK7与RCAR12在体外存在相互作用．进一步通过双分子荧光互补实验发现CARK7与RCAR12在烟草叶片表皮细胞中能产生荧光信号，说明两者在体内同样具有明显的相互作用．

英文摘要：

The ABA receptor RCARs play a key role in ABA Signal transduction process, and research on the interaction proteins of RCARs has great significance in further understanding the molecular mech- anism of plant stress resistance. The yeast two-hybrid assay showed that the yeast co-transformed of CARK7 and ABA receptor RCAR12 could grow on SD/-Trp-Leu-Ade medium. Then, CARK7 and RCAR12 were constructed into prokaryotic expression vectors pET-28a（＋） and pGEX-6p-1, respective- ly. The fusion proteins were expressed in Rosetta（DE3）and purified by affinity purification. Purified proteins were used in downstream GST-pull down assay, the result demonstrated that RCAR12 could pull down CARK7 protein. These results indicated there is a significant interaction between CARK7 and RCAR12 in vitro. Furthermore, the biomolecular fluorescence complementation （BIFC） assay showed CARK7 was associated with RCAR12 to generate fluorescence in tobacco epidermal cells, indicating that CARK7 can also interact with RCAR12 in vivo.