为了探索当归种子的休眠机制和提高其萌发率,本文以药用植物当归的种子为研究对象,分析了不同温度的水浸种不同时间和不同浓度3吲哚乙酸(IAA)溶液浸种不同时间后对当归种子萌发率的影响。结果表明:60 ℃水浸种2 min萌发率最高,为78%,是对照的2.5倍;0.05 g·L-1 IAA溶液浸种3 h萌发率最高,为80%,是对照的1.7倍;用30、35、40、45、50、55、60、65、70 ℃ 9个温度的水浸种1、2 min的萌发率与水温的相关性不显著,浸种5、10 min相关性显著,浸种15 min相关性极显著;用0.01、0.02、0.03、0.04、0.05、0.06、0.07、0.08、0.09、0.1 g·L-1 10个浓度的IAA溶液浸种1、3、6、12 h的萌发率与溶液浓度的相关性均不显著;水浸种提高种子萌发率可能与改变种子种皮透性有关,IAA溶液浸种提高种子萌发率可能与打破休眠种子的休眠有关;60 ℃水浸种2 min和0.05 g·L-1 IAA溶液浸种3 h均可显著提高当归种子的萌发率;IAA溶液为有机溶剂,污染环境,在实际生产中建议多使用水浸种法为宜。
In order to explore dormancy-breaking mechanism and increase germination percentage, the effects of different temperatures of water treatments and different concentrations of IAA (indole acetic acid) on the germination process of the seeds of Angelica sinensis were studied. The results showed that soaking seeds with IAA 0.05 g·L-1 for 3 h and soaking seeds with water 60 ℃ for 2 min had the maximum germination percentages (78% and 80%, respectively), being 1.7 and 2.5 times as high as that of the control. When soaking seeds with water 30, 35, 40, 45, 50, 55, 60, 65, 70 ℃ for 1 and 2 min, there was no significant correlation between germination percentages and temperatures, but a significant correlation was observed for 5, 10 and 15 min. There was no significant correlation between germination percentage and concentration when soaking seeds 1, 3, 6, 12 h with IAA 0.01, 0.02, 0.03, 0.04, 0.05, 0.06, 0.07, 0.08, 0.09, 0.1 g·L-1. Soaking seeds with water could improve seed germination percentage, which might relate to permeability of seed coat; however, IAA could improve seed germination percentage, which might be involved in breaking seed dormancy. Consequently, soaking seeds with water 60 ℃ for 2 min and soaking seeds with IAA 0.05 g·L-1 for 3 h could improve the germination percentages of A. sinensis seeds. Compared with water, IAA pollutes the environment easily. So, soaking seeds with water is an advisable method in actual production.