中文摘要：

目的建立Balb／C小鼠的急性哮喘模型，应用复方黄龙汤进行治疗，并与地塞米松治疗效果比较，探讨复方黄龙汤对小鼠哮喘气道炎症的影响及作用机制。方法选取24只6～8周龄雌性Balb／C小鼠，体重（20±2）g，应用随机数字法随机分为正常对照组（A组）、哮喘模型组（B组）、地塞米松治疗组（C组）和复方黄龙汤治疗组（D组），每组6只。应用卵白蛋白致敏液腹腔注射联合OVA雾化吸入法建立哮喘模型，B、C、D组在第0、14天向小鼠腹腔注射0VA配置而成的致敏液0．2ml，第28、29、30天吸入1％OVA／PBS 10m1激发哮喘，造模23天后，C组每天给予地塞来松（2mg/kg），D组每天给予复方黄龙汤（2．7ml／kg）；激发结束后，用无创肺功能测量仪测量小鼠气道反应性，qRT-PCR法测定小鼠肺组织中T-bet及GATA-3mRNA的含量，酶联吸附反应测定肺泡灌洗液中IL-4及IFN-γ含量，将肺泡灌洗液离心沉淀后涂片进行中性粒细胞、嗜酸性粒细胞和淋巴细胞等炎性细胞计数，用HE染色肺组织，评价病理改变。结果无创肺功能显示雾化吸入乙酰胆碱剂量〉12．5μg／ml时，C组和D组penh值明显低于B组（P〈0．05），但高于A组，差异无统计学意义；肺组织中T-betmRNA表达B组高于A组（P〈0．001），D组含量高于B组，但差异无统计学意义（P〉0．05）。而GATA-3mRNA表达C、D组低于B组（均P〈0．01）。肺泡灌洗液中IL-4含量B组高于A、C和D组（均P〈0．01），而IFN-γ含量B组远低于A组（均P〈0．01），D组肺泡灌洗液中IFN-γ含量较B组升高，差异有统计学意义（PGO．05）；在小鼠肺泡灌洗液中B组嗜酸粒细胞（EOS）比C、D组明显增多（P〈0．01）；B组气道壁和肺组织中大量炎性细胞浸润（以嗜酸性粒细胞、淋巴细胞为主），支气管管壁增厚，管腔狭窄，与A组比较差异明显；C、D两组小鼠细支气管管腔呈圆形，?

英文摘要：

Objective To investigate the effect and mechanism of Fu-Fang- Huang- Long Decoction on acute airway inflammation of asthmatic mice. Methods Health female Balb/C mice were randomly divided into four groups： control group （group A）, asthma group （group B）, dexamethasone group （group C） and Fu-Fang-Huang- Long Decoction group （group D）. Asthma rat model was induced by ovalbumin （OVA, via intraperitoneal injection） combined with OVA aerosol administration. After modeling, dexamethasone （2mg/kg） and Fu Fang Huang Long decoction （2.7ml/kg） was administra- ted in group C and D, respectively. The airway reactivity （AR） was determined with pulmonary function test apparatus. The expression of T-bet/GATA-3mRNA was determined using q-RT-PCR. The level of IL-4 and IFN-7 in alveolar ]avage fluid was evaluated using enzyme linked immunosorbent assay （ELISA）. Hematoxylin and Eosin （HE） staining was per- formed to evaluate the pulmonary pathologies. Results None -invasive lung ruction reveals that when chloride acetycholine dose is higher than 12.5μg/ml, the penh value in groups C and D is significantly lower than group B（P〈0. 05）, but higher than group A. There is no significant difference between group C and D. Significant decrease of T-bet mRNA expression was noted in group B compared with group A（P〈0. 001）. Though T-bet mRNA expression in group D is higher than group C, there is no significant difference between them（P〉0.05）. For the expression of GATA-3 mR.NA, remarkable increase was noted in group B compared with group C and D. Higher expression of IL-4 was noted in group B compared with the other groups （P〈0.01）. For the level of IFN-7, remarkable decrease was noted in group B compared with group A. Significant increase was noted in BALF, eosinophitic granulocytes （EOS） in group B compared with those obtained from group C and D, respectively （P~0.01）. A large number of inflammatory cells were in the airway wall and lung tissue of Group B （eo- sino