中文摘要：

目的 探讨CD166对鼻咽癌CNE-2细胞放射敏感性的影响。方法 采用流式细胞术检测鼻咽癌CNE-2细胞膜CD166阳性表达率，免疫磁珠分选技术获得CNE-2-CD166（＋）、CNE-2-CD166（－）细胞亚群，克隆形成实验验证其放射敏感性，CCK-8法及流式细胞术检测10 Gy剂量照射前后的细胞增殖率及细胞凋亡率。结果 CNE-2细胞膜CD166阳性表达率为（24.27±5.31，分选后CNE-2-CD166（＋）细胞CD166阳性表达率为96.5%，CNE-2-CD166（－）细胞为0.6%。克隆形成实验结果显示，CNE-2-CD166（－）细胞相对CNE-2-CD166（＋）细胞的放射增敏比（sensitization enhancement ratio,SER）为1.24。10 Gy照射后24 h、48 h和72 h，CNE-2-CD166（＋）细胞的存活分数分别为（（76.66±3.36）%、（62.44±1.66）%和（55.21±3.39），CNE-2-CD166（－）细胞为（60.34±5.13）%、（41.11±3.00）%和（35.28±4.36）%，同时段内两组细胞的存活分数比较，差异均有统计学意义（P＜0.05）。10 Gy照射前，CNE-2-CD166（＋）细胞和CNE-2-CD166（－）细胞凋亡率分别为（5.87±1.14）%和（6.67±1.68）%，差异无统计学意义（P＞0.05）；10 Gy照射后48 h，CNE-2-CD166（＋）细胞凋亡率为（（19.37±2.54）%，显著低于CNE-2-CD166（－）细胞的（28.90±4.04）%，差异有统计学意义（P=0.026）。结论 CD166表达阳性的鼻咽癌CNE-2细胞对放射更具抗拒性，可能与减少细胞受照射后的凋亡率降低有关。

英文摘要：

Objective The present study aimed to investigate the effect of CD166 on the radiosensitivity of nasopharyngeal carcinoma cell line CNE-2. Methods CD166 expression in the CNE-2 cell membrane was detected by flow cytometry. CNE-2-CD166（＋） and CNE-2-CD166（-） cell lines were obtained using magnetic-activated cell sorting technology. Radiosensitivity,cell proliferation,and apoptosis were assessed,respectively,using a colony formation assay,cell counting kit-8 assay and flow cytometry. Results The CD166（＋） rate in the CNE-2 cell membrane was（24.27±5.31）%;after immunomagnetic separation,the CD166（＋） rate was 96.5% among CNE2-CD166（＋） cells and 0.6% among CNE2-CD166（-） cells. The radiation sensitivity ratio was 1.24. After 10-Gy irradiation,The CCK-8 assay indicated that the survival fraction of CNE-2-CD166（＋） cells was （76.66±3.36）% in 24 h,（62.44±1.66）% in 48 h and （55.21±3.39）%in 72 h,significantly higher than that of CNE-2-CD166（-） cells：（60.34±5.13）% in 24 h、（41.11±3.00）% in 48 h,（35.28±4.36）% in 72 h（P〈0.05）. After 10-Gy irradiation,the apoptotic rate of CNE-2-CD166（＋） cells was significantly lower than that of CNE-2-CD166（-）cells（P=0.026）. Conclusion Our data suggest that CD166（＋） nasopharyngeal carcinoma CNE-2 cells are more resistant to radiation,and the mechanism may be associated with reduced apoptosis rate after irradiation.