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中文摘要：
制备了赭曲霉毒素A（OTA）单克隆抗体免疫亲和柱，并用间接竞争ELISA和HPLC法评价了免疫亲和柱的性能，其柱容量（结合OTA的能力）约为200ng，加标回收率为90．38％～100．1％，可反复使用3次。建立了免疫亲和柱-HPLC联用分析谷物中OTA的方法，最低检出限为0．2μg／kg，线性范围为0．6-400μg／kg，OTA加标量为1～10μg／kg时谷物样品中的回收率为78．7％-87．1％，变异系数小于6．5％。用此法检测了大米、小麦、玉米和玉米饲料等15份市售样品，检出率为46．7％，其中OTA的最高含量为0．785μg／kg。
英文摘要：
An immunoaffinity column （IAC） was prepared with anti-ochratoxin A （OTA） monoclonal antibody and the efficiency of IAC was analyzed by indirect competitive enzyme-linked immunosobent assay （IC-ELISA） and high performance liquid chromatography （HPLC）. Results indicated that this IAC had a high column capacity of binding OTA （200 ng） and the recovery ranging from 90.38 % to 100.1%, and could be used repeatedly 3 times. IAC linked with HPLC was used to detect OTA contents in 15 cereal samples. The limit of detection （LOD） of OTA by this method was 0.2 μ g/kg. Samples with OTA levels from 0.6 to 400 μg/kg could be quantitified by this method. The recovery of spiked OTA at levels of 1 to 10 μg/kg ranged from 78.7 % to 87.1% with coefficients of variation （CVs） less than 6.5 %. This method was applied to detect 15 saled cereal samples. The positive detection rate was 46.7 %, and the highest OTA content detected was 0.785 μ g/kg.
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