中文摘要：

目的：观察心肌成纤维细胞是否存在线粒体乙醛脱氢酶2（ALDH2）的表达,探讨ALDH2在高糖诱导的心肌成纤维细胞引起纤维化发生中的作用。方法：原代培养心肌成纤维细胞,分为正常对照组（5.5 mmol/L）、正常＋ALDH2激动剂Alda-1（20μmol/L）组、高糖组（30 mmol/L）、高糖＋Alda-1组。免疫荧光鉴定心肌成纤维细胞。各组细胞分别培养48 h后应用MTT法检测成纤维细胞增殖活力,RT-PCR和Western blot检测ALDH2 m RNA及蛋白的表达。结果：RT-PCR和Western blot结果显示心肌成纤维细胞ALDH2 m RNA和蛋白均有表达。与正常对照组相比,高糖组心肌成纤维细胞增殖能力提高（P〈0.01）,ALDH2蛋白表达下降（P〈0.05）;与高糖组相比,高糖＋Alda-1组心肌成纤维细胞增殖能力降低（P〈0.01）,ALDH2的蛋白表达增加（P〈0.05）。结论：心肌成纤维细胞存在ALDH2的表达,ALDH2激动剂Alda-1提高ALDH2的表达后可以抑制高糖引起的心肌成纤维细胞的增殖。

英文摘要：

Objective： To observe whether acetaldehyde dehydrogenase 2 （AIX）H2） is expressed in cardiac fibroblasts and investigate the change of ALDH2 in cardiac fibroblasts when cultured with high concentration of glucose. Methods： Cultured cardiac fibroblasts were randomly divided into four groups： normal control group （5.5 mmol/L glucose）, Alda-1 （the agonist of ALDH2, 20 μmol/L） group, high glucose group （30 mmol/L glucose） and high glucose ＋ Alda-1 group. Cardiac fibroblasts were identified by immunofluore-scence technique. Cell proliferation was detected by MTF method after treated with drugs for 48 hours, mRNA and protein expressions of ALDH2 were determined by RT-PCR and Western blot, aimed to ensure whether ALDH2 was expressed in cardiac fibroblasts. The changes of ALDI-I2 protein expression in cardiac fibroblasts were tested by Western blot. Results： RT-PCR and Western blot results revealed that ALDH2 was expressed in cardiac fibroblasts. Compared with normal control group, cardiac fibroblasts proliferation was increased （P 〈 0.05）, while the protein expression of ALDH2 was reduced （P 〈 0.05） in high glucose group. When treated with Alda-1, the proliferation of cardiac fibroblasts was decreased （P 〈 0.01）, while the protein expression of ALDH2 was increased （P 〈 0.05） in high glucose group. Conclusion： ALDH2 was expressed in cardiac fi- broblasts. Alda- 1, the agonist of ALDH2 enhanced the expression of ALDH2 and inhibited the proliferation of cardiac fibroblasts when cultured with high concentration of glucose.