中文摘要：

目的研究电磁辐射后大鼠海马Raf激酶抑制蛋白（RKIP）及其mRNA的动态变化规律。方法分别采用x波段高功率微波（X—HPM）、S波段高功率微波（S-HPM）及电磁脉冲（EMP）模拟源辐射大鼠．建立电磁辐射动物模型、利用免疫印迹法（westexn blot）检测RKIP的表达，反转录-聚合酶链反应（RT—PCR）检测RKIPmRNA的表达通过免疫共沉淀检测RKIP与Rat-1的相互作用，免疫组化检测脑内胆碱乙酰转移酶（CHAT）的表达。结果辐射后6h，RKIP表达明显下调，EMP组于1d恢复，两微波组于1～7d持续下州，至14d基本恢复辐射后611-7d，RKIPmRNA表达呈波浪状变化，其中6h下捌，3d上调。RKIP与Raf-1的相互作用于辐射后6h～14d均减弱，以7d最为明显，两微波组此相互作用较为明显：辐射后6h-7d，CHAT表达持续减少，至14d基本恢复。结论电磁辐射可导致Raf激酶抑制蛋白及其相关蛋白的下调。

英文摘要：

Objective To study the develupment of changes for Raf kinase inhibitor protein（RKIP） and its mRNA in rats hippncampus after electromagnetic radiation. Methods Rats were exposed to X-band high power mierowave（X-HPM）,S-band high power microwave （S-HPM） and electromagnetic pulse （EMP） radiation source respectively. The animal model of electromagnetic radiation was established. Western blot was used to detect the expression of RKIP,aod RT-PCR was applied to detect the expression of RKIP mRNA. The interaction of RKIP and Raf-1 was measured with co-immunopreeipitation method, and the expression of cere- bral choline acetyhransferase （CHAT） was measured by immunohistochemistry. Results The expression of RKIP significantly down-regulated at 6 h after radiation, and recovered at 1 d in group EMP, but the down-reg- ulation continued during 1-7 d after radiation in the two microwave groups. The expression of RKIP mRNA changed wavily during 6 h-7 d after radiation, which showed down-regulation at 6 h,and up-regulation at 3 d. The intelnction of RKIP and Raf-1 decreased during 6 h-7 d after radiation, most significantly at 7 d, and the two microwave groups were more significant. The expression of CHAT decreased continuously during 6 h-7 d after radiation,and generally recovered on 14 d, Conclusion The down-regulation of RKIP and its related proteins of hippocampus is induced by electromagnetic radiation.