中文摘要：

通过醛化法将膝沟藻毒素（GTX2，3）分别与血蓝蛋白（KLH）、牛血清白蛋白（BSA）偶联制备完全抗原，高效液相色谱法（HPLC）检测醛化反应前后游离GTX2，3的浓度，完全抗原免疫Balb／C小鼠，间接酶免疫吸附试验检测免疫后血清中抗体效价。GTX2，3与KLH及BSA偶联反应前溶液中毒素的浓度分别为760μg·mL^-1和660μg·mL^-1，偶联反应后毒素的浓度分别为191μg·mL^-1及256μg·mL^-1，Balb／C小鼠经过5次免疫后血清的效价为1：500。结果表明，反应后游离的GqN2，3的浓度降低，制备的GTX2，3完全抗原免疫小鼠后小鼠产生抗GTX2，3抗体，说明GqN2，3与载体蛋白发生偶联反应，偶联物具有免疫原性。

英文摘要：

GTX2,3 was conjugated with carrier protein and KLH GTX2,3 before and after the reaction was detected through HPLC. and BSA by the methods of formaldehyde. The concentration of Balb/C mice were immunized with GTX2,3 -KLH conjugates and the antibody titration in immunized mice serum was detected by indirect enzyme-linked immunoassay. Before the conjugation reaction, the concentration of GTX2,3 was 760μg · mL^-1 and 660μg ·mL^-1 separately before eacting with KLH and BSA. After the reaction, the corresponding concentration was 191μg · mL^-1 and 256μg·mL-1 The antibody titration against GTX2,3 in mice serum was 1： 500 after 5 times immunization. The immunogen of GTX2,3 were produced successfully through the methods of formaldehyde, and the synthesized GTX2,3 -KLH conjugates were improved for the antibody production of GTX2,3 as immunogen.