中文摘要：

目的：建立微波辅助提取、高效液相色谱同时测定山茱萸中马钱苷、獐芽菜苷及山茱萸新苷3种环烯醚萜苷的方法。方法：采用响应曲面法优化微波功率、提取溶剂以及料液比等提取条件;应用Agilent TC-C18柱分离,以水和乙腈为流动相梯度洗脱,流速1.0 mL/min,检测波长240 nm。结果：最佳微波提取工艺为：以体积分数72%的乙醇作溶剂,液料比15 mL/g,在微波功率400 W下提取10 min,连续提取两次;3个成分的高效液相色谱峰面积与浓度呈良好的线性关系（r〉0.9994）,加样回收率分别为98.68%、98.24%和98.29%。结论：所建立的微波辅助提取高效液相色谱同时测定方法简便、准确、重现性好,可用于山茱萸药材中3种环烯醚萜苷的同时测定。

英文摘要：

Objective：To establish a method of microwave-assisted extraction and high performance liquid chromatographic（HPLC） for simultaneous determination of three iridoid glycosides including loganin,sweroside and cornuside in Cornus officinalis.Methods：The extraction conditions of microwave power,ethanol concentration,liquid to sample ratio were optimized with a response surface methodology（RSM）;Three constituents were separated on an Agilent TC-C18 column by gradient elution using acetonitrile and water as the mobile phase.The flow rate was 1.0 mL/min.The detection wave length was 240 nm.Results：The optimal conditions of microwave extraction were as follows：microwave power 400 W,ethanol concentration 72%,liquid to sample ratio 15 mL/g,the extraction time 10 min,the extraction times 2;The HPLC peak areas of all the constituents showed good linearity（r〉0.9994） in the range of the tested concentration,the average recoveries of the method were 98.68%,98.24% and 98.29%,respectively.Conclusions：The established method of microwave-assisted extraction and HPLC simultaneously determination has the advantages of convenient,precision and reliability.It can be used in simultaneous determination of three iridoid glycosides in Cornus officinalis.