中文摘要：

目的调查金黄色葡萄球菌临床分离株毒力基因和耐药基因的存在及菌株系统发育分析,以降低耐药性。方法收集2013年7-9月临床标本中分离的金黄色葡萄球菌共38株,先用spa基因PCR检测用作金黄色葡萄球菌的分子鉴定,再采用聚合酶链反应（PCR）的方法分析54种毒力基因和12种耐药基因,然后做系统发育分析（N-J法）。结果金黄色葡萄球菌6类毒力基因：黏附毒素、细胞毒素、荚膜抗原、超抗原、蛋白酶、A蛋白均有检出,且检出率较高;耐β-内酰胺类、氨基糖苷类、红霉素类、四环素类、消毒剂、抗菌肽的耐药基因检出率分别为34.2%、23.7%、47.3%、2.6%、2.6%和7.9%;系统发育分析可见金黄色葡萄球菌呈明显的多态性且无相同的克隆,但多数菌株又有一定的聚集性,可分为A、B、C1及C2簇群。结论对金黄色葡萄球菌临床分离株同时检测54种毒力基因和12种耐药基因是国内首次报道,该组菌携带多种毒力基因和耐药基因是该组菌株产生致病性和耐药性的重要原因。

英文摘要：

OBJECTIVE To investigate the existence of virulence genes and resistance genes of Staphylococcus aureus of clinical isolates and to analyze the phylogeny of the strains to decrease the drug resistance. METHODS From Jul. to Sept. 2013, totally 38 strains of S. aureus were isolated from clinical samples. Then, spa was used to identify S. aureus. Furthermore, 54 kinds of virulence genes and 12 kinds of resistance genes were analyzed by PCR. Then, all genes were performed on phylogenetic analysis （N‐J） method. RESULTS A total of 6 classes of virulence genes ：adhesins, cytotoxins, capsular antigens, superantigens, proteases and Staphylococal protein A, were detected, and the detection rates were high. Moreover, the detection rate of drug resistance genes to beta‐lactam, aminoglycoside, erythromycin, tetracycline, disinfectant and antibacterial peptide were 34. 2%, 23. 7%, 47. 3%, 2. 6%, 2. 6% and 7. 9%, respectively. Phylogenetic analysis suggested that this group of strains showed obvious polymorphism and no identical clones existed. But most strains had clustering properties, and were further divided as cluster A, B, C1, C2. CONCLUSION It′s the first report that 54 kinds of virulence genes and 12 kinds of resistance genes were detected in clinical isolates of S. aureus in China. Various virulence genes and resistance genes in S. aureus played a key role in pathogenicity and resistance.