中文摘要：

运用PCR方法以保守引物NC5、NC13、NC13r和NC2扩增从内蒙古地区骆驼皱胃分离的3条斯氏副柔线虫（Parabronema skrjabini）rDNA的内转录间隔区1（ITS1）、5.8S序列和内转录间隔区2（ITS2）。将PCR扩增出的片段纯化后克隆至pGM-T载体，用PCR技术及酶切鉴定阳性菌落，对阳性菌落质粒DNA进行测序。结果表明线虫1（P.sk1）扩增的ITS片段大小为837 bp，包含部分的18S、28S及全部的ITS1（298 bp）、5.8S（157 bp）及ITS2（281 bp）序列；线虫2（P.sk2）扩增的ITS1片段大小为372 bp，包含部分的18S、5.8S及全部的ITS1（296 bp）；线虫3（P.sk3）扩增的ITS2片段大小为484 bp，包含部分的5.8S、28S及全部的ITS2（284 bp）序列。同其它属线虫同源性比较ITS2序列同源性在30.2%-60.1%。本研究系首次报道骆驼斯氏副柔线虫的ITS序列，为斯氏副柔线虫分子生物学的进一步研究奠定基础。

英文摘要：

The internal transcribed spacer （ITS1, 5.8S and ITS2）of ribosomal DNA nucleotide fragments of Parabronerna skrjabini isolated from the camel of Inner Mongolia region were amplified by PCR using three pairs of conserved primers NC5, NC13, NC13r and NC2. The PCR fragments were purified and cloned into pGM-T vector. The inserts were successfully sequenced, and the results revealed that the first samplers （P. sk1） ITS inserts were 837 bp in length and consisted of partial 18S, 28S and complete ITS1（298 bp）, 5.8S（157 bp） and ITS2 （281）rDNA sequences. The second sample＇s （P. sk2） ITS1 inserts were 372 bp in length and consisted of partial 18S, 5.8S and complete ITS1（296 bp）. The third sample＇s （P. sk3） ITS2 inserts were 484 bp in length and consisted of partial 5. 8S, 28S and complete ITS2 （284 bp）. ITS2 sequences shared 30. 2%-60.1% homology with other nematodes. It was the first time that the complete sequence of ITS and 5.8S rDNA of P. skrjabini were reported. The results of this study laid a foundation for further studies.