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中文摘要：
建立了利用Waters2695高效液相色谱测定毕赤酵母发酵液中S-腺苷蛋氨酸（S-adenosylmethionine，SAM）含量的方法，对HPLC—UV法检测SAM含量的色谱条件进行优化，最佳色谱条件如下：All-tima C18柱，150mm×4．6mm，5μm，流动相为V（0．01mmoL／L甲酸铵）：V（甲醇）=97：3，流速0．6mL／min，柱温30℃，检测波长260nm．优化后的色谱分离条件可使样品中的SAM与杂质达到较佳分离，稳定性、精密度及重复性都较好，其RSD均小于1．5％．利用SAM标品制作标准曲线Y=32408606．0721X＋130181．2824，线性范围为0．012～1．2g／L，R2达到0．9998，平均回收率为100．027％～102．533％．此方法可有效地检测发醇液中S-腺苷蛋氨酸含量．
英文摘要：
An HPLC determination method was developed for measuring S-adenosylmethionine （SAM） contents in culture broth of Pichia pastoris. The optimized HPLC conditions for SAM analysis were 0. 01 mmol/L ammonium formate solution （ pH = 3.5 ） - methylalcohol （ V： V = 97 ： 3 ） as mobile phase, flow rate 0. 6 mL/min, column temperature 30℃, and detection wavelength 260 nm. The separation conditons described above achieved good separation effect, together with high stability, sensitivity, and reproducibility. The standard curve of SAM was Y = 32408606. 0721 X ＋ 130181.2824 , and the linear range was 0. 012 × 1.2 g/L, R2 was 0. 9998, the average recycle rate was 100. 027% - 102. 533%. This method would be useful for SAM analysis during Pichia pastoris fermentation.
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