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中文摘要：
SSRs标记已成为目前应用最为广泛的一种分子标记.相较之其他棉种,海岛棉的ESTs及SSRs标记资源均相当匮乏.本研究利用本实验室2个纤维cDNA文库随机测序获得的海岛棉ESTs序列,进行海岛棉EST-SSRs分布特征分析及新SSRs标记开发与应用研究.通过对9697条非冗余ESTs序列分析,共发现SSRs位点638个,分布于595条ESTs中,EST-SSRs序列的频率是6.13%,平均相隔10.4kb出现一个SSR.在2～6bp的重复基元中,三核苷酸重复基元的SSRs出现频率最高（26.6%）,其次是六核苷酸重复（26.0%）和五核苷酸重复（25.9%）.在所有重复基元类型中所占比例最大的是（AAG）n.利用Primer3及virtualPCR程序开发SSRs引物,并去除来源于海岛棉自身部分同源序列以及与CMD释放的不同棉种冗余SSRs引物后,获得380对棉花新SSRs引物.进一步对本实验室四倍体作图亲本陆地棉TM-1和海岛棉海7124进行多态性检测,其多态率为25.8%.利用BC1作图群体,将来源于82对引物的95个多态位点定位在我室构建的海陆遗传图谱上,其中42个定位在At亚组,53个定位在Dt亚组.研究结果为进一步饱和棉花遗传图谱以及开展基于图谱的比较基因组研究奠定了基础.
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