中文摘要：

在 Arabidopsis 昆虫相互作用调查可诱导的沉香醇的角色，从有质体指向和合成 intron 的草莓的 FaNES1 沉香醇 synthase (LIS ) cDNA (李的) 被放在创伤的控制下面可诱导的朊酶禁止者 2 (PI2 ) 从土豆的倡导者。构造 pBin-PPI2-LIS' 被转变到 Arabidopsis thaliana 生态型哥伦比亚 0。卡那霉素抵抗 T0 幼苗被 PCR 分析在 RNA 上在 genomic 脱氧核糖核酸上并且由 RT-PCR 分析为李的基因的存在和抄写证实。Genomic 和 RT-PCR 产品被定序证实合成 intron 的正确拼接。由在转基因的线的 PPI2-LIS' 基因构造的活跃沉香醇 synthase 的表示被用稳固的阶段测量沉香醇排放估计微抽取(SPME ) 在有甲基 jasmonate 的正式就职以后的 GCMS 大小。在 30 根测试独立 T2 转基因的线之中， 10 展出了沉香醇生产。沉香醇表示能被甲基 jasmonate 处理，然而并非由有菱形斑纹的蛾幼虫导致。

英文摘要：

To investigate the role of inducible linalool in Arabidopsis-insect interactions, the FANES 1 linalool synthase （LIS） cDNA from strawberry with plastid targeting and a synthetic intron （LIS＇） was placed under the control of the wound inducible proteinase inhibitor 2 （PI2） promoter from potato. The construct pBin-PPi2-LIS＇ was transformed to Arabidopsis thaliana ecotype Columbia 0. Kanamycin resistant TO seedlings were confirmed for the presence and transcription of the LIS＇ gene by PCR analysis on genomic DNA and by RT-PCR analysis on RNA. Genomic and RT-PCR products were sequenced to confirm correct splicing of the synthetic intron. The expression of active linalool synthase by the PPI2-LIS＇ gene construct in the transgenic lines was assessed by measuring linalool emission using solid phase micro-extraction （SPME） GC-MS measurements after induction with methyl jasmonate. Among 30 tested independent T2 transgenic lines, 10 exhibited linalool production. Linalool expression could be induced by methyl jasmonate treatment, but not by diamondback moth larvae.