中文摘要：

我们的学习的目的是在人的 colorectal 癌在 SW620 房间增长和 apoptosis 上调查 Pin1 减小的效果的目的。为 Pin1 基因(pGenesl-1-Pin1 ) 的我们构造了 RNA 的 plasmid 介入的方法(shRNA ) ，当时， plasmid 是进房间由 liposome 衬里的 colorectal 癌 SW620 的 transfected 调停。Pin1 的蛋白质表示被西方的弄污测试。增长率被 MTT 分析，房间的 apoptotic 率被流动 cytometry 测试。为了解释，在 SW620 房间推进 Pin1 的效果， Bcl-2 的蛋白质水平被西方的弄污分析。结果 pGenesil-1-Pin1 plasmid 被定序成功地构造并且证实。Pin1 的蛋白质亲戚层次在正常人的睾丸和 seminomas 是 0.06 禁止者 PAI-1 和 PAI-2。上述基因的表达式借助于船桨被计算的方法？？

英文摘要：

Objective：The aim of our study was to investigate the effects of Pin1 reduction on SW620 cell proliferation and apoptosis in human colorectal carcinoma.Methods：We constructed a plasmid of RNA interfering（shRNA） for Pin1 gene（pGenesl-1-Pin1）,then the plasmid was transfected into colorectal carcinoma SW620 cells line by liposome mediation.The protein expression of Pin1 was tested by Western blotting.The proliferation rate was analyzed by MTT and the apoptotic rate of cells was tested by flow cytometry.In order to explain further the effect of Pin1 in SW620 cells,the protein level of Bcl-2 was analyzed by Western blotting.Results：pGenesil-1-Pin1 plasmid was successfully constructed and confirmed by sequencing.The protein relative levels of Pin1 were 0.06 ± 0.04 for the P-shRNA/SW620 cells,and 0.32 ± 0.09 for the P-Con/SW620 cells.The cell growth rate of SW620 cells was slower while the apoptotic rate was increased after transfection with pGenesil-Pin1 plasmid,and the apoptotic rate was 12.38% ± 1.55% for the P-shRNA/SW620 group.At the same time,we found that the protein expression of Bcl-2 was also reduced.The results were 0.13 ± 0.04 for the P-shRNA/SW620 cells,and 0.36 ± 0.08 for the P-Con/SW620 cells.Conclusion：Inhibited Pin1 expression may suppress the cell proliferation and promote apoptosis of colorectal carcinoma cells in vitro.