中文摘要：

目的观察未成熟髓源树突状细胞（iMDC）负载P258-73肽段诱导免疫耐受对实验性自身免疫性神经炎（EAN）的预防作用，以及对干扰素-γ（IFN-γ）、白介素-33（IL-33）mRNA表达的影响。方法（1）P258-73aa与iMDC共培养。（2）21只Lewis大鼠随机分为EAN组（A组）、iMDC组（B组）和P258-73aa-iMDC组（c组），并分别于皮下注射磷酸盐缓冲液（PBS）、iMDC及P258-73aa-iMDC；7d后，各组均给予P253-78aa和完全弗氏佐剂（CFA）进行免疫，诱发EAN。观察各组发病情况并作临床评分至免疫后16d（发病高峰期）。（3）采用^3H-TdR掺人法检测淋巴细胞增殖反应；RT—PCR检测大鼠坐骨神经、脾脏和淋巴结中IL-33、IFN-γmRNA的表达。结果（1）发病高峰期，A组、B组、C组的临床评分为（7．4±1．9）分、（5．2±1．6）分和（3．4±0．9）分，各组间比较差异有统计学意义（均P〈0．01）。（2）A组、B组、C组抗原特异性淋巴细胞增殖反应依次明显降低（均P〈0．01）。（3）A组、B组、C组坐骨神经、脾脏和淋巴结中IFN-γmRNA表达水平依次明显降低，IL-33mRNA表达依次明显增高（P〈0．05～0．01）。结论P258-73aa—iMDC能减轻EAN的发病；其可能通过抑制抗原特异性淋巴细胞增殖、降低IFN-γ分泌、上调IL-33的分泌诱导免疫耐受。

英文摘要：

Objective To observe the preventive effect of immune tolerance induced by immature myeloid dendritic cell （iMDC） loading P2 58-73 peptide （P2 58-73aa-iMDC） in experimental autoimmune neuritis （EAN） , and the effect on the expression of IFN-γ, and IL-33 mRNA. Methods （ 1 ） The iMDC were cultured in vitro for loading P2 58-73aa. （2） 21 adult female Lewis rats were randomly divided into the EAN （A）group, iMDC （B）group and P2 58-73aa -iMDC（C） group, and subcutaneously injected PBS, iMDC and P2 58-73aa-iMDC respectively. After 7 d, all the rats were immunized with P2 53-78aa and complete freunds adjuvant （CFA） to inducing EAN. Then , the situation of onset were observed and clinical score were evaluated till 16 d （the crest-time of onset ） after immunization. （3） The lymphocyte proliferative response were assayed by 3 H-TdR incorporation. The expression of IL-33, IFN-γ mRNA in sciatic nerves, spleen and lymph node were detected by RT-PCR. Results （ 1 ） In the cresttime of onset, the clinical scores in groups A,B and C were （7.4 ± 1.9）, （5.2 ± 1.6） and （3.4 ±0.9） respectively. There were significant differences between each two groups （ all P 〈 0. 01 ）. （ 2 ） The antigen specific lymphocyte proliferation in groups A, B, C were significantly decreased in proper order（ all P 〈 0. 01 ）. （ 3 ） In groups A, B and C, the expression levels of IFN-γ mRNA in sciatic nerves, spleen and lymph node were significantly reduced in proper order, while IL-33 mRNA were significantly increased in proper order （P〈 0. 05 -0. 01 ）. Conclusions P2 58-73aa-iMDC can ameliorate onset of EAN. The protective effect of it may be associated with the antigenic specific suppression of lymphocyte proliferation and reducing expression of IFN-γ,increasing the expression of IL-33 to induce immune tolerance.