以唐菖蒲品种‘RoseSupreme’为材料,研究叶片、新球、匍匐茎和籽球中蔗糖、淀粉含量及其相关酶活性变化。结果表明:唐菖蒲叶片中蔗糖含量顺序为叶片〉新球、籽球〉匍匐茎。叶片和匍匐茎中淀粉含量较新球和籽球低。叶片、新球和籽球中蔗糖磷酸合成酶(SPS)活性较高。唐菖蒲生长发育前期各部位蔗糖合成酶(SS)活性均较低,但后期新球中的ss活性快速升高,叶片中则快速下降。酸性转化酶(AI)活性的高低顺序是叶片〉匍匐茎〉籽球〉新球。叶片中性转化酶(NI)活性较其他部位高,匍匐茎不断增加,新球是先增加后降低。Q酶活性除了叶片外,其他部位均不断增加;总淀粉酶活性变化趋势均是先增加后降低。在唐菖蒲生长发育过程中,蔗糖代谢主要受SPS、SS、AI和NI的综合调控,Q酶和淀粉酶在球茎发育过程中对淀粉代谢起重要作用。
In order to determine contents of sucrose and starch and related metabolic enzyme activities, the leaves, stolons, new corms and cormels in 'Rose Supreme' were studied. The results indicated that the sequence of sucrose content is leaves 〉 new corms and cormels 〉 stolons. The starch contents of leaves and stolons were lower than new corms and cormels. Sucrose phosphate synthase (SPS) activity was higher in the leaves than stolons, except in new corms and cormels. At the early development stage of Gladiolus hybridus, the activity of sucrose synthase (SS) was lower in all parts, but ascended quickly in the new corms comparated descended in the leaves at the late stage. The sequence of acid invertase (AI) activity is leaves 〉 stolons 〉 cormels 〉 new corms. The activity of neutral invertase (NI) in leaves was higher than other parts, and stolon was ascended, whereas new corm was ascended firstly then descended. The activities of Q-enzyme increased except leaves. The activities of amylase ascended first then descended. The activities of Q-enzyme and amylase in the new corms and cormels were higher than in the leaves and stolons. From the development process of G. hybridus, sucrose-metablizing are integrately controlled by AI, NI, SPS and SS. Q-enzyme and amylase play important roles in metabolizing of starch.