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唐菖蒲2种主要病毒的多重RT-PCR检测
  • ISSN号:1001-7488
  • 期刊名称:《林业科学》
  • 时间:0
  • 分类:S682.24[农业科学—观赏园艺;农业科学—园艺学]
  • 作者机构:[1]中国农业大学观赏园艺与园林系,北京100094
  • 相关基金:国家自然科学基金资助项目(30471221).
中文摘要:

以唐菖蒲品种‘RoseSupreme’为材料,研究叶片、新球、匍匐茎和籽球中蔗糖、淀粉含量及其相关酶活性变化。结果表明:唐菖蒲叶片中蔗糖含量顺序为叶片〉新球、籽球〉匍匐茎。叶片和匍匐茎中淀粉含量较新球和籽球低。叶片、新球和籽球中蔗糖磷酸合成酶(SPS)活性较高。唐菖蒲生长发育前期各部位蔗糖合成酶(SS)活性均较低,但后期新球中的ss活性快速升高,叶片中则快速下降。酸性转化酶(AI)活性的高低顺序是叶片〉匍匐茎〉籽球〉新球。叶片中性转化酶(NI)活性较其他部位高,匍匐茎不断增加,新球是先增加后降低。Q酶活性除了叶片外,其他部位均不断增加;总淀粉酶活性变化趋势均是先增加后降低。在唐菖蒲生长发育过程中,蔗糖代谢主要受SPS、SS、AI和NI的综合调控,Q酶和淀粉酶在球茎发育过程中对淀粉代谢起重要作用。

英文摘要:

In order to determine contents of sucrose and starch and related metabolic enzyme activities, the leaves, stolons, new corms and cormels in 'Rose Supreme' were studied. The results indicated that the sequence of sucrose content is leaves 〉 new corms and cormels 〉 stolons. The starch contents of leaves and stolons were lower than new corms and cormels. Sucrose phosphate synthase (SPS) activity was higher in the leaves than stolons, except in new corms and cormels. At the early development stage of Gladiolus hybridus, the activity of sucrose synthase (SS) was lower in all parts, but ascended quickly in the new corms comparated descended in the leaves at the late stage. The sequence of acid invertase (AI) activity is leaves 〉 stolons 〉 cormels 〉 new corms. The activity of neutral invertase (NI) in leaves was higher than other parts, and stolon was ascended, whereas new corm was ascended firstly then descended. The activities of Q-enzyme increased except leaves. The activities of amylase ascended first then descended. The activities of Q-enzyme and amylase in the new corms and cormels were higher than in the leaves and stolons. From the development process of G. hybridus, sucrose-metablizing are integrately controlled by AI, NI, SPS and SS. Q-enzyme and amylase play important roles in metabolizing of starch.

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期刊信息
  • 《林业科学》
  • 北大核心期刊(2011版)
  • 主管单位:中国科协
  • 主办单位:中国林学会
  • 主编:尹伟伦
  • 地址:北京万寿山后中国林学会
  • 邮编:100091
  • 邮箱:lykx@vip.sina.com
  • 电话:010-62889820
  • 国际标准刊号:ISSN:1001-7488
  • 国内统一刊号:ISSN:11-1908/S
  • 邮发代号:82-6
  • 获奖情况:
  • 在三届"国家期刊奖"评选中,两次荣获中国期刊最高奖-"国家期刊奖",一次名列"国家期刊奖提名奖"第一名
  • 国内外数据库收录:
  • 俄罗斯文摘杂志,美国化学文摘(网络版),英国农业与生物科学研究中心文摘,波兰哥白尼索引,美国工程索引,美国剑桥科学文摘,英国动物学记录,日本日本科学技术振兴机构数据库,中国中国科技核心期刊,中国北大核心期刊(2004版),中国北大核心期刊(2008版),中国北大核心期刊(2011版),中国北大核心期刊(2014版),中国北大核心期刊(2000版)
  • 被引量:42472