中文摘要：

以E.coli为微生物催化剂,新亚甲基蓝为电子媒介体,葡萄糖为燃料,铁氰化钾为电子受体,构建介体型微生物燃料电池（MFC）实验装置;采用在线驯化法,即MFC产电和槽内E.coli驯化的联合过程,以提高E.coli电化学活性,该法无须刮移生物膜,操作简便。结果表明,驯化后E.coli产电能力得到显著提高,在E.coli干重为1.24 mg/mL时,MFC峰电流密度达到612.50mA/m^2,较未被驯化E.coliMFC提高了0.54倍;最大功率输出达到166.67 mW/m^2,提高了0.64倍;同时MFC生物膜成熟所需时间减少至240 min,比未被驯化时加快近一倍。

英文摘要：

A mediator microbial fuel cell （MFC） was constructed by using E. coli as biocatalyst, new methylene blue as electron mediator, glucose as electron donor, and potassium ferricyanide as the electron acceptor. Online taming method was adopted to improve the electrochemical activity of E. coli, namely a combination of taming E. coli and generating electricity simultaneously in the same MFC, which did not need to scrap off the biofilm of MFC. The electricity generation of the tamed E. coli MFC was compared with the electricity generation of the parental E. coli MFC when the initial concentration of E. coli cells was 1.24 mg dry cell weight per milliiter, the tamed E. coli MFC showed a 54% improvement in peak current density, which reached to 612.50mA/m^2; a 64% improvement in the maximum power output, which reached to 166.67mW/m^2 ,meantime,the tamed biofilm＇s maturation time was obviously reduced to 240 min, quickened up 1 times compared with the parental E. coli＇s.