中文摘要：

以大量的室内模拟培养实验，以内蒙古温带草甸草原土壤为研究对象，利用AIM乙炔抑制法，模拟野外条件对原样土壤样品进行N2O产生过程进行研究。实验结果表明：内蒙古温带草甸草原土壤N2O产生过程以硝化作用为主。其中异养硝化作用起主导作用，自养硝化潜势和反硝化潜势在草原植物不同生长季节变化不同，总体上异养硝化潜势〉白养硝化潜势〉反硝化潜势。由于自养硝化作用在不同季节的发生，使得草甸草原土壤N2O的产生潜势也高、低起伏变化。从而揭示了内蒙古温带草原土壤以异养硝化作用过程为主产生N2O和N2O排放通量较低的微生物学机理。

英文摘要：

In this paper, A lots of laboratory experiments using the acetylene inhibition method （AIM） were carried to investigate the N2O production processes in chernozem soil of meadow grassland in Inner Mongolia. The research results showed that the heterotrophic nitrification is the important microbiological process of N2O production in this kind soil. The potential of autotrophic nitrification and denitrification are different with the changing of grassland vegetation＇s growing periods. However, the sequence of their contribution is： heterotrophic nitrification 〉 autotrophic nitrification 〉 denitrification. The increasing and decreasing of N2O production potential result from the appearance of autotrophic nitrification in different growing period. Thereby, the N2O emission flux from temperate grassland soils was lower because that the heterotrophic nitrification is key N2O production process in these soils.