中文摘要：

分别对反相、正相、离子交换、体积排阻及亲和等色谱柱的清洗与再生方法加以系统综述.使用适当的方法对受污染的色谱柱进行清洗与再生,可恢复其部分分离能力,延长使用寿命.对硅胶基质反相色谱柱,可选择一系列溶剂,按洗脱能力逐渐增强的顺序依次冲洗,或者使用二甲基甲酰胺、乙酰丙酮、SDS及盐酸胍等强洗脱试剂清洗;硅胶基质正相色谱柱用极性逐渐增强的溶剂系统冲洗;有机聚合物、石墨碳及氧化锆固定相色谱柱,用稀酸、稀碱和有机溶剂结合清洗,可将受污染的色谱柱柱效提高50%以上.

英文摘要：

Methods for cleaning and regeneration of reversed phase,normal phase,ion exchange,size exclusion chromatographic,affinity chromatographic and other liquid chromatographic columns are summarized in this paper,aiming at the recovery of the separation ability of contaminated columns,and the extension of the lifetime of columns.According to the sequence of the increase of solvent strength a series of solvents can be used for,the cleaning and regeneration of silica-based reversed-phase chromatographic columns,and some strong solvents such as MMF,SDS,acetone and hydrochloric acid cna also be used.For silica-based normal phase chromatographic columns a solvent washing system with increased polarity is effective.The recommended column washing solvents for organic polymers,graphite carbon and zirconium oxide stationary phase columns are dilute acid,dilute alkali and organic solvents.After cleaning and regeneration by these methods,the column efficiency of contaminated columns can be increased by 50%.