中文摘要：

根据草莓psy、pds和zds基因序列，设计含有酶切位点的特异引物，以草莓总RNA为模板，通过RT-PCR法分别克隆psy、pds和础基因．以植物表达载体pBI121为基础，利用口蹄疫病毒2A序列将psy、pds和zds基因融合在同一个开放阅读框下，构建成三价融合植物双元表达载体pBI2A2Apsy-pds-zds.同时构建了psy和础基因二价融合植物表达载体pBI2A2Apsy-zds．经PCR、限制性内切酶酶切和测序鉴定后，成功将2个重组表达质粒pBI2A2Apsy-pds-zds和pBI2A2Apsy-zds导入农杆菌EHA105中．

英文摘要：

Specific primers containing different enzyme sites were designed on the basis ofpsy, pds and zds genes sequence of straw- berry. With the template of total RNA, psy, pals and zds genes were amplified by RT-PCR. Based on the vector of pBI121, the tri- valent plant express vector pBI2A2Apsy-pds-zds containing psy, pds and zds genes and fused in a single open reading frame （ORF） by foot and mouth disease virus （FMDV） was constructed. In addition, the divalent expression vector pBI2A2Apsy-zds containing psy and zds genes was also constructed. Vectors of pBI2A2Apsy-pds-zds and pBI2A2Apsy-zd, s were checked by PCR, restriction en- zymes analysis and sequencing, and transformed into Agrobactrium tumefaciens EHA 105.