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土壤中萘降解菌的耐铅性能及机制初步研究
  • ISSN号:1001-5914
  • 期刊名称:《环境与健康杂志》
  • 时间:0
  • 分类:R124[医药卫生—环境卫生学;医药卫生—公共卫生与预防医学] R117[医药卫生—公共卫生与预防医学]
  • 作者机构:[1]天津理工大学化学化工学院,天津300384, [2]天津市疾病预防控制中心卫生检测所理化室
  • 相关基金:国家自然科学基金青年基金(31100400);天津市应用基础及前沿技术研究计划(15JCQNJC08800);国家级大学生创新训练项目(201610060037)
中文摘要:

目的探讨土壤中萘降解菌对萘和重金属双重污染的耐受性。方法对油田石油污染土壤中分离到的一株高效萘降解菌——伯克霍尔德菌(Burkholderia sp.)TN的重金属耐受性、铅富集机制及在土壤生物修复中的应用进行研究,以分光光度法测定不同Pb2+浓度(100500 mg/L)对菌悬液吸光度的影响,以电感耦合等离子体发射光谱仪(ICP-OES)测定培养基中重金属(50 mg/L,Pb2+,Zn2+,Cu2+和Cd3+)在菌体培养液、细胞壁、细胞内富集物中的分布,以扫描电镜(SEM)、能谱仪(EDS)和傅里叶变换红外光谱(FTIR)观察100 mg/L Pb2+对菌体形态、元素及官能团分布的影响。结果菌株TN对Pb2+的最高耐受浓度为500 mg/L并可耐受一定浓度的Zn2+、Cu2+和Cd3+,TN对Pb2+富集程度最高,细胞壁及胞内富集率达到98.33%;菌体蓄积Pb2+后细胞壁表面有沉淀物附着并有铅元素检出,对Pb2+的蓄积主要依靠细胞壁上的羧基、羟基、酰胺基及磷酸基团;将TN菌株接种到含有2.5 mg/g萘及200 mg/L Pb2+的灭菌土壤中,经过13 d室温培养之后,萘的去除率为94.29%。结论 TN菌株对Pb2+、Zn2+、Cu2+和Cd3+均具有一定的耐受性,对Pb2+的富集能力强。该菌株能够依靠细胞壁上的活性基团富集Pb2+,适用于萘和Pb2+双重胁迫的土壤修复。

英文摘要:

Objective To explore the lead tolerance of a naphthalene-degrading strain TN isolated from oil contaminated soil of oil field. Methods A naphthalene-degrading strain TN isolated from oil contaminated soil of oil field was collected for research on the resistance to different heavy metals, the mechanism of lead accumulation and application in soil bioremediation.The mechanism of lead accumulation by stain TN was evaluated by inductively coupled plasma-optical emission spectrometry(ICPOES), scanning electron microscopy-energy dispersive X-ray spectroscopy(SEM-EDS) and infrared spectroscopy(FTIR).Results The results of resistance experiments showed that the maximum tolerated concentration of lead was 500 mg/L and could tolerate a certain concentration of Zn2+, Cu2+and Cd3+. The adsorption rate of Pb2+was the highest(98.33%) by cell wall and intracellular. The granular lead was precipitated on the cell wall of bacteria based on the possible functional groups such as carboxyl, hydroxyl, amide group and phosphoric acid group. After inoculating strain TN into the autoclaved soils containing0.25% naphthalene and 200 mg/L Pb2+, incubating it at room temperature for 13 days, 94.29% naphthalene was removed.Conclusion TN strain has a certain resistance to heavy metals such as Pb2+, Zn2+, Cu2+and Cd3+. This strain can enrich Pb2+by the active groups on the cell wall and better effect of degradation was realized by applying to the remediation of naphthalene and Pb2+contaminated soil.

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期刊信息
  • 《环境与健康杂志》
  • 中国科技核心期刊
  • 主管单位:国家卫生和计划生育委员会
  • 主办单位:天津市疾病防控制中心 中华预防医学会
  • 主编:王撷秀
  • 地址:天津市河东区华越道6号
  • 邮编:300011
  • 邮箱:hjyjk@263.net
  • 电话:022-24333577
  • 国际标准刊号:ISSN:1001-5914
  • 国内统一刊号:ISSN:12-1095/R
  • 邮发代号:6-221
  • 获奖情况:
  • 中华预防医学会系列杂志优秀期刊,天津市一级期刊
  • 国内外数据库收录:
  • 美国化学文摘(网络版),英国农业与生物科学研究中心文摘,波兰哥白尼索引,美国剑桥科学文摘,日本日本科学技术振兴机构数据库,中国中国科技核心期刊,中国北大核心期刊(2004版),中国北大核心期刊(2008版),中国北大核心期刊(2011版),中国北大核心期刊(2014版),中国北大核心期刊(2000版)
  • 被引量:20716