中文摘要：

目的：人偏肺病毒（human metapneumovirus,hMPV）是婴幼儿呼吸道感染重要的病毒病原,目前尚无有效预防和治疗药物。本研究探讨玉屏风散对小鼠感染hMPV的保护作用。方法：将40只BALB/c小鼠随机分为5组,实验组经不同剂量玉屏风散灌胃14d后,滴鼻感染荧光标记的重组hMPV。感染后第5天处死小鼠,无菌状态下取肺组织,左肺用于病理切片,RT-PCR法检测hMPV感染;右肺称重,按每克肺组织加入10mL鼠肺组织缓冲液,4℃匀浆,通过改良噬斑形成法检测肺组织匀浆中hMPV滴度。结果：①HE染色提示大剂量玉屏风散组比病毒对照组小鼠肺组织炎症程度显著减轻[病理评分（5.13±0.84）vs（15.25±1.49）,P〈0.01];②病毒对照组、小剂量组、中剂量组、大剂量组肺内平均病毒滴度分别为（2.33±0.98）×10^5、（2.08±0.11）×10^5、（5.08±1.46）×104、（2.17±0.75）×10^2 PFU/g,中、大剂量组与病毒对照组比较肺内病毒滴度与肺组织病理评分呈正相关（r=0.844,P〈0.01）。结论：大剂量玉屏风散预防可显著抑制hMPV在小鼠肺内复制水平,减轻肺组织病理损害。玉屏风散预防病毒感染的机制是以直接抗病毒作用抑或调节机体抗病毒免疫功能为主尚待进一步研究。

英文摘要：

Objective： HMPV has been clearly demonstrated to be the syncytial virus（RSV）.However,there has been no vaccine and validated therapeutics available by far for this important virus.This study investigated the prophylactic effects of a Chinese traditional prescription,Yupingfeng Powder,in mice from infection of hMPV.Methods： Forty BALB/c mice were randomly divided into 5 groups.All the mice except those in mock-infection group were inoculated intranasally with hMPV after being orally lavaged with various doses of Yupingfeng Powder for 2 weeks.The animals were sacrificed on day 5 post inoculation.Left lungs were harvested for pulmonary histopathology and detection of hMPV infection by PCR.Right Lungs were used for viral isolation and titration by a modified plaque assay.Results： ①The prophylactic use of high dose Yupingfeng Powder dramatically reduced pulmonary histopathology[pathological score（5.13±0.84）vs（15.25±1.49）,P〈0.01].The pathological scores were well paralleled by viral titers in the lungs.②High dose Yupingfeng Powder was capable of reducing lung viral titer as much as 3 logs（2.33±0.98）×105、（2.08±0.11）×105、（5.08±1.46）×104、（2.17±0.75）×102PFU/g.Conclusion： Yupingfeng Powder appears to be potent inhibitor for hMPV replication in mouse lungs.Whether Yupingfeng Powder has direct antivirus or immune regulatory effects,however,is unclear at this point.