中文摘要：

目的 探讨燃煤型砷中毒患者9年前后遗传损伤变化与病情进展关系.方法 1998年2月追踪观察贵州省兴仁县燃煤型砷中毒病区确诊的206例砷中毒患者,分为可疑、轻度、中度、重度砷中毒4个组;另观察病区12 km外非砷污染的健康人群67名.经过9年随访,2006年12月将生化指标完整的131例砷中毒患者（可疑、轻度、中度、重度砷中毒组分别有17、39、42、33例）及45名对照纳入研究.采用二乙基硫代氨基甲酸银分光光度法（Ag-DDC）检测尿砷、发砷含量;采用遗传学方法检测外周血淋巴细胞微核（MN）率和染色体畸变（CA）率,采用单细胞凝胶电泳法（SCGE）检测DNA单链断裂,以彗星尾长表示DNA损伤程度.结果 2006年对照可疑、轻度、中度、重度砷中毒组的尿砷[分别为（34.16±10.25）、（52.35±22.41）、（62.26±31.13）、（71.43±49.92）和（78.45±50.64） μg/L]和发砷[分别为（1.37±0.56）、（3.69±1.78）、（4.88±3.49）、（5.21 ±3.10）和（6.25±4.04） μg/g]含量均低于9年前的尿砷[分别为（36.07±20.70）、（73.65±41.33）、（90.92±82.14）、（126.55±107.31）和（139.44±90.90） μg/L]和发砷[分别为（1.41±1.18）、（4.85±4.20）、（5.72±4.07）、（6.43±4.32）和（7.19±4.68） μg/g],其差异均有统计学意义（F值分别为10.63、7.72、14.66、11.00,P值均＜0.05）.2006年可疑、轻度、中度、重度砷中毒组MN率[分别为（0.238±0.130）％、（0.268 ±0.192）％、（0.283±0.157）％和（0.391±0.233）％]、CA率[分别为（14.36±5.44）％、（18.09 ±6.49）％、（19.38 ±5.63）％和（19.83±5.84）％]和DNA彗星尾长[分别为（29.88±13.81）、（29.84±12.80）、（34.50±9.88）和（41.58±12.98） μm]均高于9年前MN率[分别为（0.163 ±0.051）％、（0.186±0.117）％、（0.196±0.104）％和（0.273 ±0.142）％]、CA率[分别为（13.18 ±5.17）％、（14.48 ±6.61）％、（15.67 ±8.49）％和?

英文摘要：

Objective To investigate the changes of genetic damage in patients with arsenism caused by coal-burning in 9 years.To analyze the relationship between the changes of genetic damage and disease progression and provide a basis for condition monitoring.Methods Of 206 arsenism patients from the area with endemic arsenism in Guizhou province were tracking surveyed in February 1998 and divided into 4 groups,including suspicious,mild,moderate and severe poisoning group.Another 67 healthy residents from a neighbour township 12 km away where arsenic was not prevalent were surveyed.Over a 9-year follow-up,131 arsenism patients and 45 controls with the complete biochemical indexes among them were selected as subjects in December 2006.Arsenic （As） concentration of urine and hair were detected by silver diethvldithiocarbamate spectrophotometry （Ag-DDC）.Micronucleis （MN） and chromosome aberrations （CA） were analyzed by conventional methods.DNA single-strand breaks of peripheral blood were measured by single cell gel electrophoresis （SCGE）,and the tail lengths of comet were used to measure DNA damage.Results Among the control,suspicious,mild,moderate and severe arsenic poisoning group,the As contents of urine and hair were respectively （34.16 ± 10.25）,（52.35 ±22.41）,（62.26 ±31.13）,（71.43 ±49.92）,（78.45 ± 50.64） μg/L and （1.37 ±0.56）,（3.69 ± 1.78）,（4.88 ± 3.49）,（5.21 ± 3.10）,（6.25 ± 4.04） μg/g in 2006,which were lower than that 9 years before （urine as contents were （36.07 ± 20.70）,（73.65 ± 41.33）,（90.92 ± 82.14）,（126.55 ± 107.31） and （139.44 ± 90.90） μg/L,and hair As contents were （1.41 ± 1.18）,（4.85 ± 4.20）,（5.72 ± 4.07）,（6.43 ± 4.32） and （7.19 ±4.68） μg/g,respectively,F value was 10.63,7.72,14.66,11.00 respectively,all P values were ＜ 0.05）.Except for suspicious poisoning group,the differences of urine As contents in the other groups all showed significance （P ＜ 0.05）.The incidences of MN wer