中文摘要：

构建表达载体PBI121-AtDGK7，转化拟南芥（Arabidopsis thaliana）获得过表达转基因植株。通过抗逆生理指标测定、ABA、NaCI和甘露醇抗性试验，以及半定量RT-PCR方法，对拟南芥甘油二酯激酶（AtDGK7）基因的抗逆生理功能进行初步研究。结果表明，过表达AtDGK7转基因拟南芥对高浓度ABA的敏感性比野生型要低，能更好地抵御盐胁迫。通过测定脯氨酸（Pro）和丙二醛（MDA）含量以及超氧物歧化酶（SOD）活性，进一步证实过表达AtDGK7转基因拟南芥植株的耐低温能力增强。

英文摘要：

Transgenic AtDGK7 plants were obtained by constructing PBI121-AtDGK7 expression vector and transformed into Arabidopsis thaliana. And the stress-resistant function of diacylglycerol kinase （AtDGK7） gene in Arabidopsis thaliana was researched by stress resistance determination, ABA, NaC1 and mannitol resistance experiment and RT-PCR. The results showed that the At- DGK7-overexpressed Arabidopsis thaliana plants were less sensitive to high concentration ABA and displayed normal germination of seeds. Under salt stress, the AtDGK7-overexpressed Arabidopsis thaliana plants grew more robust than that of the wild-type Arabidop- sis thaliana plants, and could resist the salt stress. Meanwhile, the free proline and malonaldehyde contents, and superoxide dismutase activities were determined. And the results showed that the low temperature-resistant of the AtDGK7-overexpressed Arabidopsis thaliana plants were improved.