中文摘要：

目的：探讨中药复方菩人丹（PRD）对OLETF大鼠视网膜中自杀相关因子（fas）和半胱天冬酶-8（caspase-8）表达的干预作用。方法：将24只成模的OLETF大鼠随机分为PRD治疗组、糖尿病模型组，每组均为12只，同周龄12只雄性LETO大鼠为正常对照组，PRD治疗组大鼠连续灌胃PRD，1．8g／kg，1次／d，2个月。HE染色观察大鼠视网膜的形态结构改变；TUNEL法检测OLETF大鼠视网膜神经细胞的凋亡情况；免疫组化法和Western Blot方法检测OLETF大鼠视网膜fas和caspase-8表达的蛋白；RT-PCR方法检测各组大鼠视网膜中fas和caspase-8 mRNA的表达情况。结果：与正常对照组LETO大鼠相比，OLETF模型组的大鼠视网膜出现了明显的病理变化，神经细胞的凋亡指数、fas、caspase-8的蛋白及mRNA表达明显升高（P〈0．01）。与模型组大鼠相比，PRD治疗组大鼠视网膜的病理变化明显减轻，神经细胞的凋亡指数、fas、caspase-8蛋白及mRNA表达明显降低（P〈0．01）。结论：PRD可通过抑制fas、caspase-8的表达，减少糖尿病大鼠视网膜神经细胞的凋亡，从而起到保护糖尿病视网膜神经组织的作用。

英文摘要：

Objective： To investigate the interventions of traditional chinese medicine PRD on the factors associated suicide （fas） and cysteine aspartic acid specific protease-8 （caspase-8）in retina of the OLETF rats. Methods：24 rats were randomly divided into two groups, a PRD treatment group and a diabetes model group and 12 male LETO rats of same age as a normal control group, PRD 1.8 g/kg （l/d） was intragastrically administrated to the rats of PRD treatment group for 2 months. Then rats retinal morphology were observed by Hematoxylin-eosin staining and apoptosis of OLETF rat retinal neurons were detected by TUNEL. Besides, immunohistochemical staining and Western Blot were used to detect caspase-8 and fas in protein level. RT-PCR was used to detect caspase-8 and fas in mRNA level. Results：Compared with normal control LETO group, the neuronal apoptosis index, the protein and mRNA expression of fas and caspase-8 of dia- betes model OLETF group were significantly increased （ P 〈 0.01 ）, but compared with the model group, neuronal apoptosis index, the protein and mRNA expression of fas and caspase-8 protein in the PRD treatment group were significantly decreased （P 〈 0.01 ）. Conclusion：PRD can inhibit fas and caspase-8 expression, reducing neuronal apoptosis in diabetic rat retina, which play a protective role in diabetic retinal nerve tissue.