中文摘要：

目的探讨ICU中重症肺炎患者肺泡灌洗液中微小RNA（MicRNA）表达与重症肺炎的关系。方法采用MicRNA芯片技术对5例重症肺炎患者和5例非呼吸道感染术后患者的肺泡灌洗液MicRNA进行筛选，最终筛选出其中上调表达、下调表达的MicRNA；再通过RT—PCR的方法，验证上述芯片检测的结果。结果建立重症肺炎患者肺泡灌洗液的MicRNA异常表达谱，从中筛选出40个上调表达的MicRNA和113个下调表达的MicRNA。通过与非呼吸道感染患者对比，重症肺炎组上调表达基因miR-3686、miR-4455及下调表达基因miR-1323、miR-127—5p差异有统计学意义（P〈0．05）。结论miR-3686、miR-4455、miR-1323、miR-127—5p在重症肺炎患者的肺泡灌洗液中明显表达，提示其与重症肺炎的发生有一定的相关性。

英文摘要：

Objective To observe the expressions of MicRNA in bronchoalveolar lavage fluid of patients with severe pneumonia in Intensive Care Unit （ICU）. Methods The differential expressions of MicRNA in BALF of patients with SP （ n = 5 ） and non - SP patients （5 eases who needed mechanical ventilation after surgery） were screened by MicRNA chip technique. Screen out the MicRNA which were up - regulated and down - regulated and the results were verified by RT - PCR chip. Results Abnormal expression spectrum of MicRNA in severe pneumonia patients was initially built,in which 40 MicRNA were up - regulated and 113 MicRNA were down - reg- ulated. Compared with non - SP patients, the expressions of miR - 3686, miR - 4455, miR - 1323 and miR - 127 - 5p were significant difference in SP patients than those in non - SP patients （ P 〈 0. 05 ）. Conclusion The expressions of miR - 3686, miR - 4455, miR - 1323 and miR -127 -5p are significant difference in BALF of patients with severe pneumonia,which may relate to pulmonary injury.