中文摘要：

采用PCR方法对本实验室分离保存的10株禽波氏杆菌、3株兔支气管败血波氏杆菌及1株猪支气管败血波氏杆菌菌株,扩增其16SrRNA基因的5′端片段（783bp）,并测定所得片段的DNA序列。用DNAStar分析软件将所获得的序列与GenBank中的禽波氏杆菌序列进行比较,由此构建禽波氏杆菌菌株的系统发育树。结果显示,10株禽波氏杆菌核苷酸序列与GenBank中收录的AF177666株禽波氏杆菌核苷酸序列的同源性为82.0%～82.5%,与兔支气管败血波氏杆菌和猪支气管败血波氏杆菌核苷酸序列的同源性均为99.2%～99.9%,其中P9（山鸡波氏杆菌）、P11（兔支气管败血波氏杆菌）与P14（猪支气管败血波氏杆菌）分别发生1个核苷酸的缺失。本试验结果表明,16SrRNA序列分析是鉴定禽波氏杆菌的一种快速而准确的方法。

英文摘要：

To establish the sequence analysis of 16S rRNA of Bordetella avium for the identification of Bordetella avium strains.The 5＇-end fragments（783 bp） of 16S rRNA genes were amplified from ten Bordetella avium strains,three rabbit Bordetella bronchiseptica strains and one porcine Bordetella bronchiseptica strain,which were isolated and preserved from the author＇s lab,by PCR and the DNA fragments were directly sequenced. The sequences obtained in this study were compared with the Bordetella avium 16S rRNA sequence reported in GenBank by software of DNAStar analyses.Results showed the homology between ten Bordetella avium strains and the reported strain in GenBank were 82.0%-82.5%,and were 99.2%-99.9% identical to the three rabbit Bordetella bronchiseptica strains and one porcine Bordetella bronchiseptica strain.The pheasant Bordetella avium strain, one rabbit Bordetella bronchiseptica strain and the porcine Bordetdlla broachiseptica strain deleted one nucleotide respectively. It is concluded that 16S rRNA sequence analyses may he a reliable and rapid way for identification of Bordetella avium;Bordetella avium strains isolated from the civil were especially different with those isolated from other countries on the genotypes.