中文摘要：

传统的蛋白酶解方法往往需要过夜，这大大限制了蛋白质的分析速度．微波辅助化学反应方法以其快速（一般只需要十几分钟）和节能的特点已经在化学领域得到广泛应用．微波辅助酶解蛋白近几年逐渐成为该领域的研究热点．微波酶解蛋白质的方法大多采用微波炉H0和CEM公司生产的微波消解系统．目前，微波辅助酶解蛋白的操作条件主要通过质谱检测进行调整，实验过程中样品的处理费时，操作复杂，仪器的维护费用高．我们采用本课题组构建的一种新的微波辅助酶解蛋白装置，

英文摘要：

The mini microwave resonance cavity was used to accelerate proteolytic cleavage of protein. A fluorescent polymer PPESO3 as a fluorescent indicator was used to monitor proteolytic cleavage of protein in the microwave assisted protein digestion process. In this paper, the long-term stability of the PPESO3 and the PPESO3 mixed with trypsin was studied, and the detection of proteolytic cleavage of bovine serum albumin （BSA） was obtained satisfactory results. The PPESO3 was provided a novel, simple, quick determination to set the optimization parameter of the microwave-assisted protein hydrolysis. Meanwhile, the fluorescent detection devices can be integrated into the microwave assisted protein digestion system, for the development of the mini Low-power microwave protein proteolytic system would provide a very good idea.