中文摘要：

目的探讨IGF-1在酒精性肝病伴发肠粘膜损伤中的作用，并观察谷氨酰胺对肠粘膜IGF-1表达的影响。方法将30只小鼠随机分为对照组、模型组和治疗组，每组10只。对照组饲喂Lieber—Decarli无酒精液体饲料，模型组和治疗组饲喂含4％乙醇Lieber-Decarli液体饲料建立酒精性肝病模型，治疗组同时给予谷氨酰胺灌胃。12周后处死小鼠，心脏采血检测血清内毒素；取肠组织进行肠形态学检查，采厣免疫组织化学法检测IGF-1表达。结果模型组血清内毒素较对照组明显升高（0．38±0．05Eu／L对0．13±0．02Eu／L，P〈0．05），治疗组较模型组血清内毒素水平下降；模型组结肠损伤病理学评分较对照组明显升高（10．3±1．3分对4．8±1．2分，P〈O．05），治疗组结肠损伤病理学评分较模型组降低；模型组肠粘膜IGF-1表达明显高于对照组（2.3±0．2分对0．9±0．2分），治疗组表达也相应的减少。结论酒精性肝病时伴有肠粘膜损伤。IGF-1在酒精性肝病伴发肠粘膜损伤中起到了修复作用。谷氨酰胺可保护肠粘膜，而且可能与IGF—l的表达有关。

英文摘要：

Objective To study the effect of IGF-1 on intestinal mucous injury in mice with alcohol- induced liver disease and to observe the impact of glutamine on expression of IGF-1. Methods Thirty c57 mice were randomly divided into control,model and intervention group （10 in each）. The control group were fed with Lieber Decarli liquid without ethanol,and mice in the model and intervention group were fed with 4% ethanol Lieber Decarli liquid. At the same time,glutamine was given to the mice in the intervention group. The serum endotoxin was examined after 12 weeks,and intestinal tissues were assessed with HE staining and the expression of IGF-I in intestine was detected by immunohistochemical method. Results The serum endotoxin in the model group was much higher than that in control group（0.38±0.05 Eu/L vs. 0.13±0.02 Eu/L,P〈0.05）,while it decreased in intervention group as compared with the model group;the histopathological score of colon injury in the model group was much higher than that in control group （10.3±1.3 vs 4.g±l.2,P〈O.O5）;the expression of IGF in the model group was much higher than that in control group （2.3±0.2 vs. 0.9±0.2,P〈0.05）. Conclusion Alcoholic liver disease is always complicated with intestinal mucous injury. IGF-1 might play a role in alcoholic liver disease. Glutamine can protect the intestinal mucosa,and it may be associated with the expression of IGF-1.