中文摘要：

白细胞衍生趋化因子2（1eukocytecell．derivedchemotaxin2，LECT2）是一个参与多种生理和病理过程的分泌型细胞因子。该文采用毕赤酵母表达体系分泌表达虹鳟LECT2，用阳离子交换柱结合分子筛层析方法分离纯化目的蛋白，并获得纯度为96％，得率为120me／t,的重组虹鳟（Oncorhynchusmykiss）LECT2酵母培养物。生物学活性验证表明该重组蛋白能趋化虹鳟头肾来源的巨噬细胞，增强其呼吸爆发和杀菌能力，并改变其细胞因子等基因的表达。综上，该实验建立了一种快速有效的虹鳟LECT2活性重组蛋白的制备方法，为后续相关蛋白的功能研究奠定了基础。

英文摘要：

Leukocyte cell-derived chemotaxin 2 （LECT2） is a secretory cytokine that functions in many physiological and pathological processes. We useda Pichia pastoris expression system for the recombinant expression of rainbow trout LECT2. The recombinant LECT2 was purified by UNOsphere S Cation exchange and size-exclusion chromatography columns. The obtained target protein was highly pure （〉96% homogeneity） and the yield was 〉120 mg/L of yeast cultures. An in vitro chamber assay revealed that recombinant LECT2 could induce chemotactic responses in rainbow trout head kidney-derived macrophages. Recombinant LECT2 not only enhanced macrophage respiratory burst activity and bactericidal activity, but also changed macrophage gene expression. In summary, we established a rapid and efficient method to prepare active recombinant rainbow trout LECT2 using a yeast expression system and column chromatography. Bioactive recombinant LECT2 is essential for studies on protein functions.