中文摘要：

拟南芥和烟草在植物分子生物学研究中都占有非常重要的地位，为了后续试验的顺利进行，欲探索适合提取拟南芥及烟草幼嫩种子总RNA的经济快捷的方法。采用4种方法分别对拟南芥和烟草幼嫩种子的总RNA进行了提取和分析，3种方法所提取的总RNA均能满足一般的下游操作。Trizol法提取的RNA质量低。CTAB-异丙醇法提取的总RNA产量最高，并具有提取步骤少，操作时间短，价格便宜等优点，可用于大量样品提取；百泰克试剂盒提取时间短，可在常温下操作；CTAB—LiCl法提取的总RNA基本无基因组DNA的污染，但是不利于小分子RNA的沉淀。经紫外分光光度计检测，3种方法提取的总RNAOD260/OD230。均在1．80～1．97，表明蛋白质及其他有机溶剂的污染很少，OD260/OD230在2．03～2．37，表明盐类小分子的污染也较少；电泳检测28SrRNA和18SrRNA条带清晰，28SrRNA的亮度基本为18SrRNA的2倍。所提取的总RNA质量较高。将CTAB．异丙醇法提取的拟南芥总RNA用于反转录，可获得高质量的cDNA，dscDNA清晰的分布在100~3000bp之间。

英文摘要：

Total RNA was extracted from immature seed of Arabidopsis thaliana and Nicotiana tobaccum using four different methods. Trizol reagent resulted in low quantity and poor quality of RNA. The other three meth- ods could isolate RNA from immature seed with quality that match the downstream experiments. Among these methods, CTAB-Isopropyl alcohol method could get the highest yield of total RNA, with the advantages of time saving and low cost. Bioteke kit was also a convenient method to isolate immature seed RNA under room tem- perature. CTAB-LiC1 method could get higher quality total RNA without genome DNA contamination. Howev- er, LiC1 couldn＇t precipitate small molecule RNA well. The quality of total RNA isolated using the above three methods was analyzed with UV spectrophotometer and agarose gel electrophoresis. The OD260/OD230 ratio was 1.80-1.97 and OD260/OD230 ratio was 2.03-2.37. 28S rRNA and 18S rRNA were clearly detected in the RNA electrophoresis. Total RNA isolated by these methods was reverse transcribed and high quality cDNAs were obtained.