中文摘要：

目的：探讨在异戊烯焦磷酸（IPP）作用下,健康人外周血和新生儿脐带血中γδT细胞的增殖和亚型变化,以期获得足够的具有不同特征的γδT细胞用于后续的实验研究。方法：流式细胞术分析经IPP诱导的单个核细胞表面分子的表达状态,评估其γδT细胞的比例、亚型和表型格局。结果：健康人外周血和新生儿脐带血中γδT细胞数量虽占较低比例,但两者的γδT细胞存在显著异质性。健康人外周血γδT细胞主要以Vγ9Vδ2 TCR亚群为主;初始分选的外周血单个核细胞中Vγ9Vδ2T细胞主要为中央记忆型（CD27＋CD45RA-）和效应记忆型（CD27-CD45RA-）;IPP诱导后,γδT细胞显著扩增,Vγ9Vδ2 T细胞亚群转变为以效应记忆型为主,并高表达HLA-DR和B7分子。而新生儿脐带血来源的γδT细胞却呈现出较为复杂的亚群异质性,其Vγ9Vδ2 T细胞主要为CD27＋CD45RA＋的幼稚型细胞;经IPP诱导14天后,γδT细胞获得扩增（比例增高）且Vγ9Vδ2 T细胞趋向中央记忆型和效应记忆型分化,但仍以幼稚型为主。结论：健康人外周血和新生儿脐带血中γδT细胞在数量、亚群诸方面存在差异;IPP具有诱导和扩增外周血γδT细胞的生物学作用,而脐带血来源的γδT细胞还需相关细胞因子的协同作用,才能呈现出其分化成熟为可用于理论研究和临床实验的效应记忆型γδT细胞的潜能。关于其具有的免疫调节和效应功能将另文报道。

英文摘要：

Objective：To investigate the variation of γδ T cells from healthy human peripheral blood（PB） and neonatus cord blood（CB） in proliferation and subtypes with isopentenyl pyrophosphate（IPP）,and to acquire enough γδ T cells possessing distinct characteristics for experimental study.Methods：Mononuclear-cells from peripheral blood and cord blood induced by IPP were stained separately with monoclonal antibodies,which were fluorescein-labeled,and then used for assaying the expressing condition of surface molecules,as well as to evaluate the variation of γδ T cells on the percentage,subtypes and phenotypes by FCM.Results：γδ T cells only account for a small proportion in both PB and CB.However,there was a significant difference in the heterogeneity between peripheral blood and cord blood γδ T cells.Vγ9Vδ2 T cells were dominant in peripheral blood γδ T cells.Most of Vγ9Vδ2 T cells in fresh isolated PBMC were central memorytype（CD27＋CD45RA-） and effector memory-type（CD27-CD45RA-） with IPP,PB γδ T cells proliferated strongly;The effector memory-type（CD27-CD45RA-） turned into the main subtype in all Vγ9Vδ2 T cells,and HLA-DR and B7 molecules were highly expressed on the populations.But the cord blood γδ T cells showed rather complex subgroup heterogeneity,and Vγ9Vδ2 T cells were almost nave-type（CD27＋CD45RA＋）;Though γδ T cells were expanded（the percent of γδ T cells was increased）,and Vγ9Vδ2 T cells were differentiated towards central memory-type and effector memory-type on day 14 with IPP,most of γδ T cells still remained in the phase of nave-type（CD27＋CD45RA＋）.Conclusion：There lies great differences of γδ T cells in quantity and subtypes between healthy person peripheral blood and neonatus cord blood.Peripheral blood γδ T cells can be activated and proliferated with IPP,while cord blood γδ T cells have the potential to deferentiate into effector memory-type which can be used for experimental and clinical study with the synergy o