中文摘要：

层次被发现了在许多植物伴随多重不能生活的压力环境适应的不饱和的丰满的酸(FA ) 的修正。三角洲 12 丰满的酸 desaturase (FAD2 ) 由把油的酸(18:1 ) 变换成罂酸的酸(18:2 ) 在植物房间在多元不堡和船边交货的合成起一个关键作用。更好理解在多元不堡和船边交货新陈代谢和压力改编之间的关系，在在在 Arabidopsis thaliana 的各种各样的不能生活的压力和植物激素处理下面的 FA 作文的 FAD2 基因和变化的表示在这研究被调查。FAD2 基因的 1 个 423-bp 倡导者从 Arabidopsis 被克隆并且描绘。几通常认为的荷尔蒙 -- 并且 stressinducible cis 元素在克隆的倡导者被识别，它包括表明相关 WRKY71OS 的盐可诱导、病原体可诱导的 GT-1 主题， low-temperature-responsive MYC 元素，脱水应答的 MYB 元素，和 GA 元素。调查 FAD2 基因的好规定， recombinant FAD2 promoter-GUS 构造被介绍进 Arabidopsis 工厂。组织化学的学习证明倡导者在 Arabidopsis 幼苗不仅对包括骆驼毛的织物， 24-eBL，和 SA 的外长的植物激素而且到黑暗，温度，盐，和蔗糖压力无所不在地积极、应答。与表示变化，有外长的 24-eBL 的处理，骆驼毛的织物， SA，和 NaCl 一致在 Arabidopsis 幼苗在多元不堡和船边交货导致了减小。这些调查结果建议有在它的倡导者的许多通常认为的反应元素的 FAD2 基因对多重植物激素和不能生活的压力应答可以因此在工厂生长和种子开发期间在 Arabidopsis 的压力回答起一个重要作用。

英文摘要：

Modification of unsaturated fatty acid （FA） levels has been found to accompany multiple abiotic stress acclimations in many plants. Delta 12 fatty acid desaturase （FAD2） plays a critical role in the synthesis of polyunsaturated FAs in plant cells by converting oleic acid （18：1） to linoleic acid （18：2）. To better understand the relationship between polyunsaturated FAs metabolism and stress adaptation, the expression of FAD2 gene and changes in the FA compositions under various abiotic stresses and phytohormone treatments in Arabidopsis thaliana was investigated in this study. A 1 423-bp promoter of the FAD2 gene was cloned and characterized from Arabidopsis. Several putative hormone- and stress- inducible cis-elements were identified in the cloned promoter, which include salt- and pathogen-inducible GT-1 motifs, low-temperature-responsive MYC element, dehydration-responsive MYB element, and GA signaling related WRKY71OS element. To investigate the fine regulation of FAD2 gene, a recombinant FAD2 promoter-GUS construct was introduced into Arabidopsis plants. Histochemical study showed that the promoter was ubiquitously active and responsive not only to exogenous phytohormones including ABA, 24-eBL, and SA but also to darkness, temperature, salt, and sucrose stresses in Arabidopsis seedlings. Consistent with the expression change, treatments with exogenous 24-eBL, ABA, SA, and NaCl resulted in reduction in polyunsaturated FAs in Arabidopsis seedlings. These findings suggest that the FAD2 gene with a wide variety of putative response elements in its promoter is responsive to multiple phytohormones and abiotic stresses and therefore may play an important role in stress responses of Arabidopsis during plant growth and seed development.