为了解延边黄牛体细胞克隆早期胚胎发育过程中端粒酶活性的变化规律,采用实时荧光定量PCR法检测体细胞克隆早期胚胎及卵母细胞端粒酶活性。研究结果表明,端粒酶活性在未成熟卵母细胞相对水平较高,2-细胞至16-细胞胚胎的端粒酶活性总体水平相对较低(P〈0.05),桑椹胚的端粒酶活性总体水平显著升高(P〈0.05),在囊胚阶段达到最高。
The objective of this study was to examine the telomerase activity in early embryo cloned from somatic cells of Yanbian cattle by real-time PCR.The results were as follows:the telomerase activity in immature oocyte was higher,that of 2-cell~16-cell is lower than that of the others(P0.05). Telomerase activity increased significantly in the morula stage (P0.05),the highest was in the blastocyst stage.