中文摘要：

目的探讨人胰岛素对体外培养的大鼠肝细胞BRL-3A增殖和凋亡的影响。方法人胰岛索作用于BRL-3A后，用MTT法检测细胞活力，流式细胞仪检测细胞凋亡和细胞周期，qRT—PCR检测相关基因的表达。结果人胰岛素呈剂量依赖性地促进BRL-3A增殖（P〈0．05或P〈0．01）；500nmol／L人胰岛素处理3d后，处于G0／G1期的细胞比例显著下降（P〈0．05）；促凋亡基因BAX表达下凋（P〈0．05），而促细胞增殖基因CCNA2表达上调（P〈0．05）。结论人胰岛素可通过下调BAX表达和上调CCNA2表达，从而抑制大鼠肝细胞BRL-3A凋亡，促进细胞增殖。

英文摘要：

Objective To study the effect of human insulin on cell cycle progression and apoptosis of rat liver cell line BRL-3A in vitro. Methods MTF method was used to observe the effect of insulin on cell activity, and flow eytometry was used to detect cell apoptosis and cell cycle, qRT-PCR was used to evaluate the expression of related genes. Results Human insulin induced the proliferation of BRL-3A cells in a dose-dependent manner （P 〈0.05 or P 〈0.01 ） ; After 3 days treated by human insulin （500 nmol/L） , the proportion of cells in G0/Gl phases re- markably decreased （P 〈 0.05 ）. Moreover, pro-apoptotic BAX was down-regulated （P 〈 0.05 ）, while cell prolif- eration-related gene CCNA2 was up-regulated （P 〈 0. 05 ）. Conclusions Human insulin may inhibit the apoptosis of BRL-3A cell line and induce proliferation due to the down-regulated expression of BAX and up-regulated expres- sion of CCNA2.