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Cellulase in Anoplophora glabripennis adults emerging from different host tree species
  • 时间:0
  • 分类:S76[农业科学—森林保护学;农业科学—林学]
  • 作者机构:[1]Key Laboratory for Silviculture and Conservation of Ministry of Education, Beijing Forestry University, Beijing 100083, P. R. China, [2]Horticulture Administration Bureau of Wuzhong City, Ningxia Province, Wuzhong 751100, P. R. China
  • 相关基金:Acknowledgements This study was supported by the National Natural Science Foundation of China (Grant No. 30571503) and the Program for Changjiang Scholars and Innovative Research Team in Universities (IRT0607). The results presented here were developed based on previous research projects. We therefore acknowledge the role of our collaborators GAO Pei-ji in the cellulase research and YIN You-ping, SUO Feng-mei, LI Qing and CHEN Min in longhorn beetle research. We also thank especially CHEN Min for technical guidance.
  • 相关项目:光肩星天牛成虫寄主选择记忆效应的研究
中文摘要:

以便在害虫调查绗维素酶的不同类型,我们选择了从四不同宿主种出现的 Anoplophora glabripennis (Motschulsky ) 的男、女的成年人(Populus 白长袍的 var。金字塔形, P。nigra var。thevestina (Dode ) 豆子, P。simonii × P。pyramidliscr cv。歌剧 8277 Hsu。并且 Salix matsudana f。lobato-glandulosa ) 作为我们的研究材料。在成年昆虫的肠的三种绗维素酶的酶 activitives 被测量。Cx 绗维素酶 isozymes 与合并 CMC 的聚丙烯酰胺胶化被检测。结果显示出那:从不同主人种类出现的男性和女性的酶活动之间没有统计上重要的差别。在活动的数量级是:Cx 绗维素酶 】 β-glycosidase 】 C 1-cellulase。当成年人从一样的宿主种出现时, β-glycosidase 和 C 1-cellulase, 的男、女的酶活动之间没有统计上重要的差别,但是男性的 Cx 绗维素酶的酶活动清楚地比女性的高。从不同主人树出现的男性和女性的 Cx 绗维素酶 isozymes 的模式和移植清楚地不是不同的,并且任何一个都不是他们不同当从一样的主人出现时。

英文摘要:

In order to investigate different kinds of cellulase in insect pests, we selected male and female adults of Anoplophora glabripennis (Motschulsky) emerging from four different host species (Populus alba var. pyramidalis, P. nigra var. thevestina (Dode) Bean., P. simonii × P. pyramidliscr cv. Opera 8277 Hsu. and Salix matsudana f. lobato-glandulosa) as our research material. The enzyme activitives of three kinds of cellulase in the intestines of the adult insects were measured. The Cx-cellulase isozymes were detected with a CMC-incorporated polyacrylamide gel. The results show that: there are no statistically significant differences between the enzyme activities of males and females emerging from the different host species. The order of magnitude in activity is: Cx-cellulase 〉 β-glycosidase 〉 C1-cellulase. When the adults emerge from the same host species, there are no statistically significant differences between male and female enzyme activities of β-glycosidase and C1-cellulase, but the enzyme activity of Cx-cellulase of males is clearly higher than that of females. The patterns and migration of Cx-cellulase isozymes of males and females emerging from differ-ent hosts trees are clearly not different, and neither are they different when emerging from the same hosts.

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