中文摘要：

目的建立一种同时测定调更汤（白芍、地黄、首乌藤、淫羊藿等）中芍药苷、2,3,5,4’-四羟基二苯乙烯-2-O-β-D-葡萄糖苷与淫羊藿苷的反相高效液相色谱-二极管阵列检测器法。方法采用Kromasil-C18色谱柱（250 mm×4.6 mm,5μm）,以乙腈-水为流动相,梯度洗脱,体积流量1 mL/min,柱温30℃,芍药苷检测波长230 nm,2,3,5,4’-四羟基二苯乙烯-2-O-β-D-葡萄糖苷检测波长320nm,淫羊藿苷检测波长270nm。结果芍药苷、2,3,5,4’-四羟基二苯乙烯-2-O-β-D-葡萄糖苷、淫羊藿苷线性范围分别为12.1～193.4μg（r=0.999 9）,12.8～204.2μg（r=0.999 7）,22.1～353.0μg（r=0.999 9）。平均加样回收率（n=9）分别为99.89%,101.35%,97.83%;RSD分别为4.1%,1.3%,1.1%。结论该法准确、快速、重复性良好,适用于调更汤的质量控制。

英文摘要：

AIM To use HPLC coupled with photo diode array detector to simultaneously detremine the contents of three major constituents in Tiaogen Decoction,including paeoniflorin,2,3,5,4＇-tetrahyoxystilbene-2-O-β-D-glucoside,and icariin. METHODS The analysis was performed on a kromasil-C18column with mobile phase using acetonitrile and water in gradient elution manner. Three wavelengths at 230,320,and 270 nm were chosen as the monitoring wavelength to determine three active components. RESULTS The calibration curves were linear in the ranges of 12. 1- 193. 4 μg for paeoniflorin（ r = 0. 999 9）,12. 8- 204. 2 μg for 2,3,5,4＇-tetrahyoxystilbene-2-O-β-D-glucoside（ r = 0. 999 7）,22. 1- 353. 0 μg for icariin（ r = 0. 9999）. The average recoveries（ n =9） were 99. 89%,101. 35%,97. 83% and RSDs were 4. 1%,1. 3%,1. 1%,respectively. CONCLUSIONS The developed assay could be considered as a suitable quality control method for Tiaogeng Decoction.