中文摘要：

粉棒束孢是一种重要的昆虫病原真菌.运用ISSR分子标记研究了安徽省6个不同地理环境的粉棒束孢种群遗传异质性.结果表明：10个多态性引物多态位点百分率高达98.5%,但种群水平的多态位点差异较大,在59.6%～93.2%.基于Nei遗传异质性分析得出各种群间的遗传分化系数（Gst）为0.3365,基因流（Nm）为0.4931;各种群间的遗传分化低于种群内的遗传分化,表明安徽省粉棒束孢的遗传变异主要存在于种群内.根据各菌株间的遗传相似系数进行UPGMA聚类,结果表明,西山种群是单系的同质种群,其余5个种群皆为多系的异质种群,其中鹞落坪种群的异质性最高,琅琊山种群异质性最低.各种群之间的地理距离与遗传距离之间无相关关系.根据6个种群间的遗传距离进行UPGMA聚类,可将它们分成3个类群,分类的结果与各种群的地理环境相符,反映出环境的异质性对种群异质性的影响.

英文摘要：

Isaria farinosa is an important entomopathogenic fungus.By using ISSR,this paper studied the genetic heterogeneity of six I.farinosa populations at different localities of Anhui Province,East China.A total of 98.5% polymorphic loci were amplified with ten polymorphic primers,but the polymorphism at population level varied greatly,within the range of 59.6%-93.2%.The genetic differentiation index（Gst） between the populations based on Nei＇s genetic heterogenesis analysis was 0.3365,and the gene flow（Nm） was 0.4931.The genetic differentiation between the populations was lower than that within the populations,suggesting that the genetic variation of I.farinosa mainly come from the interior of the populations.The UPGMA clustering based on the genetic similarities between the isolates revealed that the Xishan population was monophylectic,while the other five populations were polyphylectic,with the Yaoluoping population being the most heterogenic and the Langyashan population being the least heterogenic.No correlations were observed between the geographic distance and the genetic distance of the populations.According to the UPGMA clustering based on the genetic distance between the populations,the six populations were classified into three groups,and this classification was accorded with the clustering based on geographic environment,suggesting the effects of environmental heterogeneity on the population heterogeneity.